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Literature summary extracted from

  • Jovcic, B.; Venturi, V.; Topisirovic, L.; Kojic, M.
    Inducible expression of choline sulfatase and its regulator BetR in Pseudomonas sp. ATCC19151 (2011), Arch. Microbiol., 193, 399-405.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.6 gene betC and DNA of betC and betR intergenic region, DNA and amino acid sequence determination and analysis, the betC promoter is inducible Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.6 additional information construction of an enzyme-deficient strain ATCC 19151DELTAbetC and its complementation for choline sulfate utilization by using pLAFRCS cosmid that carries complete choline sulfate operon Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.6 choline-O-sulfate + H2O Pseudomonas sp.
-
 choline + sulfate
-
 ?
3.1.6.6 choline-O-sulfate + H2O Pseudomonas sp. ATCC 19151
-
 choline + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.6 Pseudomonas sp. D8LAR6 isolate from sewage with the ability to degrade detergents, e.g. SDS, gene betC
-
3.1.6.6 Pseudomonas sp. ATCC 19151 D8LAR6 isolate from sewage with the ability to degrade detergents, e.g. SDS, gene betC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.6 choline-O-sulfate + H2O
-
 Pseudomonas sp. choline + sulfate
-
 ?
3.1.6.6 choline-O-sulfate + H2O
-
 Pseudomonas sp. ATCC 19151 choline + sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.6 BetC gene name, the BetC protein belongs to alkPPc superfamily and shares CXPXR sequence with the cysteine sulfatases of group I Pseudomonas sp.
3.1.6.6 choline sulfatase
-
 Pseudomonas sp.

Expression

EC Number Organism Comment Expression
3.1.6.6 Pseudomonas sp. transcription of betC and betR is not induced in the presence of high concentration of NaCl additional information
3.1.6.6 Pseudomonas sp. the putative transcriptional regulator BetR activates betC and betR transcription in the presence of choline sulfate, whilst in the absence of choline sulfate BetR represses its own transcription. BetR directly binds to betC-betR intergenic region in vitro, with higher affnity in the presence of choline sulfate as cofactor up

General Information

EC Number General Information Comment Organism
3.1.6.6 metabolism genetic regulation of the enzyme expression, overview Pseudomonas sp.