EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.3 | diphosphatidyl glycerol | activates the enzyme with the optimum concentration at 0.04 mg/ml | Vibrio alginolyticus | |
7.1.1.3 | liponox DCH | the activity is saturated at 0.01-0.02% (v/v) Liponox DCH | Vibrio alginolyticus | |
7.1.1.3 | additional information | the enzyme is not activated by sodium cholate, phosphatidyl glycerol, phosphatidyl ethanolamine and soybean phospholipids | Vibrio alginolyticus | |
7.1.1.3 | n-dodecyl octaethyleneglycol monoether | effective activator | Vibrio alginolyticus | |
7.1.1.3 | N-lauroylsarcosine | effective activator | Vibrio alginolyticus | |
7.1.1.3 | Zwittergent 3-14 | effective activator | Vibrio alginolyticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.3 | 2-n-heptyl-4-hydroxyquinoline N-oxide | - |
Vibrio alginolyticus | |
7.1.1.3 | KCN | - |
Vibrio alginolyticus | |
7.1.1.3 | ZnSO4 | - |
Vibrio alginolyticus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.1.1.3 | membrane | - |
Vibrio alginolyticus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.3 | Cu2+ | the oxidase contains copper atoms | Vibrio alginolyticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
7.1.1.3 | 13000 | - |
1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE | Vibrio alginolyticus |
7.1.1.3 | 36000 | - |
1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE | Vibrio alginolyticus |
7.1.1.3 | 79000 | - |
1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE | Vibrio alginolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.1.1.3 | Vibrio alginolyticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.1.1.3 | DEAE-Sephacel gel filtration, DEAE-5PW gel filtration, and Sephacryl S-300 gel filtration | Vibrio alginolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.1.1.3 | duroquinol + O2 + H+/in | - |
Vibrio alginolyticus | duroquinone + H2O + H+/out | - |
? | |
7.1.1.3 | additional information | horse heart reduced cytochrome c is not oxidized by this enzyme | Vibrio alginolyticus | ? | - |
? | |
7.1.1.3 | N,N,N',N'-tetramethylphenylene diamine + O2 | - |
Vibrio alginolyticus | ? | - |
? | |
7.1.1.3 | ubiquinol-1 + O2 + H+[side 1] | - |
Vibrio alginolyticus | ubiquinone-1 + H2O + H+[side 2] | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.1.1.3 | heterodimer | 1 * 79000 + 1 * 36000 + 1 * 13000, SDS-PAGE | Vibrio alginolyticus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.1.1.3 | cytochrome bo-type ubiquinol oxidase | - |
Vibrio alginolyticus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
7.1.1.3 | cytochrome | the oxidase contains cytochrome b, cytochrome o | Vibrio alginolyticus | |
7.1.1.3 | heme | the purified oxidase contains both protoheme and heme O | Vibrio alginolyticus |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
7.1.1.3 | 0.0032 | - |
in 25 mM potassium phosphate (pH 7.5), at 30°C | Vibrio alginolyticus | 2-n-heptyl-4-hydroxyquinoline N-oxide | |
7.1.1.3 | 0.0034 | - |
in 25 mM potassium phosphate (pH 7.5), at 30°C | Vibrio alginolyticus | ZnSO4 | |
7.1.1.3 | 0.0064 | - |
in 25 mM potassium phosphate (pH 7.5), at 30°C | Vibrio alginolyticus | KCN |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.1.1.3 | physiological function | the bo-type ubiquinol oxidase is functioning as a proton pump | Vibrio alginolyticus |