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Literature summary extracted from

  • Katayama, K.; Kobayashi, T.; Oikawa, H.; Honma, M.; Ichihara, A.
    Enzymatic activity and partial purification of solanapyrone synthase: First enzyme catalyzing Diels-Alder reaction (1998), Biochim. Biophys. Acta, 1384, 387-395.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.3.42 solanapyrone synthase activity is rather unstable in both crude and partially purified states. It loses 55% of its activity after 36 h at 4°C but the addition of 30% glycerol markedly improves stability, the preparation retains 55% activity for 9 days at 4°C. Other additives such as EDTA, thiol reagents and reducing agents are not effective Alternaria solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.42 0.016
-
 prosolanapyrone II in 50 mM potassium phosphate buffer, pH 7.0, at 30°C Alternaria solani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.42 additional information the activity of solanapyrone synthase is not affected by divalent cations Alternaria solani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.42 46000 62000 native enzyme, gel filtration Alternaria solani
5.5.1.20 46000 60000 gel filtration Alternaria solani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.20 prosolanapyrone III Alternaria solani the enzyme is involved in solanapyrone biosynthesis (-)-solanapyrone A
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.42 Alternaria solani
-
-
-
5.5.1.20 Alternaria solani D7UQ40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.42 partial purification by ammonium sulfate precipitation and DEAE-Sepharose column chromatography Alternaria solani
5.5.1.20
-
 Alternaria solani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.5.1.20 mycelium
-
 Alternaria solani
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.42 0.00084
-
 crude extract, in 50 mM potassium phosphate buffer, pH 7.0, at 30°C Alternaria solani
1.1.3.42 0.0206
-
 after 25fold purification, in 50 mM potassium phosphate buffer, pH 7.0, at 30°C Alternaria solani

Storage Stability

EC Number Storage Stability Organism
1.1.3.42 4°C, crude and partially purified states in the presence of 30% glycerol, 9 days, 45% loss of activity Alternaria solani
1.1.3.42 4°C, crude and partially purified states, 36 h, 55% loss of activity Alternaria solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.42 prosolanapyrone II + O2
-
 Alternaria solani prosolanapyrone III + H2O2
-
 r
5.5.1.20 prosolanapyrone III the enzyme is involved in solanapyrone biosynthesis Alternaria solani (-)-solanapyrone A
-
 ?
5.5.1.20 prosolanapyrone III the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A Alternaria solani (-)-solanapyrone A in an enzyme-catalyzed Diels–Alder reaction the product prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.42 solanapyrone synthase
-
 Alternaria solani
1.1.3.42 SPS
-
 Alternaria solani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.20 30
-
 assay at Alternaria solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.42 5.5
-
 in sodium acetate buffer Alternaria solani
1.1.3.42 7
-
 in potassium phosphate buffer Alternaria solani
1.1.3.42 7.5
-
 in Tris buffer Alternaria solani
5.5.1.20 7
-
-
 Alternaria solani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.20 5 9 pH 5.0: about 55% of maximal activity, pH 9.0: about 55% of maximal activity Alternaria solani

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.42 3.7
-
 irreversible inactivation is observed below pH 3.7 Alternaria solani
5.5.1.20 3.7
-
 irreversible inactivation below Alternaria solani

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.42 additional information the activity of solanapyrone synthase is not affected by NAD+, NADP+, FMN, and FAD+ Alternaria solani

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.5.1.20 Alternaria solani isoelectric focusing
-
 4.25