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Literature summary extracted from

  • Syrjaenen, L.; Tolvanen, M.; Hilvo, M.; Olatubosun, A.; Innocenti, A.; Scozzafava, A.; Leppiniemi, J.; Niederhauser, B.; Hytoenen, V.P.; Gorr, T.A.; Parkkila, S.; Supuran, C.T.
    Characterization of the first beta-class carbonic anhydrase from an arthropod (Drosophila melanogaster) and phylogenetic analysis of beta-class carbonic anhydrases in invertebrates (2010), BMC Biochem., 11, 28.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.1.1 drug development Drosophila beta-CA represents a highly active mitochondrial enzyme that is a potential model enzyme for anti-parasitic drug development Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 gene DmBCA, phylogenetic analysis, expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 5-acetamido-1,3,4-thiadiazole-2-sulfonamide
-
 Drosophila melanogaster
4.2.1.1 Br-
-
 Drosophila melanogaster
4.2.1.1 Cl-
-
 Drosophila melanogaster
4.2.1.1 ClO4- poor inhibition Drosophila melanogaster
4.2.1.1 CN-
-
 Drosophila melanogaster
4.2.1.1 CNO-
-
 Drosophila melanogaster
4.2.1.1 F-
-
 Drosophila melanogaster
4.2.1.1 H2NSO2NH2
-
 Drosophila melanogaster
4.2.1.1 H2NSO3H
-
 Drosophila melanogaster
4.2.1.1 HCO3-
-
 Drosophila melanogaster
4.2.1.1 HS-
-
 Drosophila melanogaster
4.2.1.1 HSO3-
-
 Drosophila melanogaster
4.2.1.1 I-
-
 Drosophila melanogaster
4.2.1.1 N3-
-
 Drosophila melanogaster
4.2.1.1 NO2-
-
 Drosophila melanogaster
4.2.1.1 NO3-
-
 Drosophila melanogaster
4.2.1.1 Ph-AsO3H2
-
 Drosophila melanogaster
4.2.1.1 Ph-B(OH)2
-
 Drosophila melanogaster
4.2.1.1 SCN-
-
 Drosophila melanogaster
4.2.1.1 SO42-
-
 Drosophila melanogaster
4.2.1.1 sulfamide
-
 Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.1 mitochondrion
-
 Drosophila melanogaster 5739
-
4.2.1.1 additional information subcellular localization study, overview Drosophila melanogaster
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Drosophila melanogaster
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Drosophila melanogaster
-
 gene DmBCA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Drosophila melanogaster CO2 + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.1 dimer dynamic light scattering analysis and gel filtration Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 beta-CA
-
 Drosophila melanogaster
4.2.1.1 beta-carbonic anhydrase
-
 Drosophila melanogaster
4.2.1.1 CO2 hydratase
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 20
-
 assay at Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 950000
-
 CO2 pH 8.3, 20°C Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 8.3
-
 assay at Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.000049
-
 5-acetamido-1,3,4-thiadiazole-2-sulfonamide pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 0.15
-
 sulfamide pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 0.15
-
 H2NSO2NH2 pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 0.67
-
 CN- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 0.73
-
 CNO- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 0.8
-
 F- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 0.97
-
 Cl- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 1.01
-
 HS- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 1.04
-
 Br- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 1.12
-
 N3- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 1.18
-
 I- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 1.28
-
 SCN- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 1.29
-
 HSO3- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 1.36
-
 SO42- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 2.45
-
 H2NSO3H pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 22.4
-
 Ph-B(OH)2 pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 26.9
-
 HCO3- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 28.6
-
 NO2- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 32.6
-
 Ph-AsO3H2 pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 43.7
-
 NO3- pH 8.3, 20°C Drosophila melanogaster
4.2.1.1 200
-
 ClO4- above, pH 8.3, 20°C Drosophila melanogaster

General Information

EC Number General Information Comment Organism
4.2.1.1 evolution phylogenetic analysis of beta-class carbonic anhydrases in invertebrates, overview Drosophila melanogaster

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.1 110000
-
 CO2 pH 8.3, 20°C Drosophila melanogaster