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Literature summary extracted from
- Glutamate racemization and catabolism in Fusobacterium varium (2011), FEBS J., 278, 2540-2551.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.1 | additional information | charcoal adsorbs coenzyme B12 and inactivates glutamate mutase | Fusobacterium varium |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.1 | Co2+ | essential constituent of coenzyme B12 | Fusobacterium varium |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 20000 | - |
gel filtration | Fusobacterium varium |
| 5.1.1.3 | 29000 | - |
calculated from amino acid sequence | Fusobacterium varium |
| 5.4.99.1 | 15000 | - |
x * 15000 + x * 51000, about | Fusobacterium varium |
| 5.4.99.1 | 51000 | - |
x * 15000 + x * 51000, about | Fusobacterium varium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.1 | L-threo-3-methylaspartate | Fusobacterium varium | - |
L-glutamate | - |
? | |
| 5.4.99.1 | L-threo-3-methylaspartate | Fusobacterium varium NCTC 10560 / ATCC 8501 | - |
L-glutamate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.2 | Fusobacterium varium | - |
- |
- |
| 5.1.1.3 | Fusobacterium varium | - |
- |
- |
| 5.1.1.3 | Fusobacterium varium NCTC 10560 / ATCC 8501 | - |
- |
- |
| 5.4.99.1 | Fusobacterium varium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.3 | ammonium sulfate precipitation | Fusobacterium varium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.3 | D-glutamate | - |
Fusobacterium varium | L-glutamate | - |
? | |
| 5.1.1.3 | D-glutamate | - |
Fusobacterium varium NCTC 10560 / ATCC 8501 | L-glutamate | - |
? | |
| 5.1.1.3 | L-glutamate | - |
Fusobacterium varium | D-glutamate | - |
? | |
| 5.1.1.3 | L-glutamate | - |
Fusobacterium varium NCTC 10560 / ATCC 8501 | D-glutamate | - |
? | |
| 5.4.99.1 | L-threo-3-methylaspartate | - |
Fusobacterium varium | L-glutamate | - |
? | |
| 5.4.99.1 | L-threo-3-methylaspartate | equilibrium constants favouring glutamate over 3-methylaspartate, formation of L-threo-(2S,3S)- and L-erythro-(2S,3R)-3-methylaspartate in the reverse reaction direction | Fusobacterium varium | L-glutamate | no activity with D-glutamate | ? | |
| 5.4.99.1 | L-threo-3-methylaspartate | - |
Fusobacterium varium NCTC 10560 / ATCC 8501 | L-glutamate | - |
? | |
| 5.4.99.1 | L-threo-3-methylaspartate | equilibrium constants favouring glutamate over 3-methylaspartate, formation of L-threo-(2S,3S)- and L-erythro-(2S,3R)-3-methylaspartate in the reverse reaction direction | Fusobacterium varium NCTC 10560 / ATCC 8501 | L-glutamate | no activity with D-glutamate | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.1 | ? | x * 15000 + x * 51000, about | Fusobacterium varium |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.1 | Glutamate mutase | - |
Fusobacterium varium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.3 | additional information | the enzyme is cofactor-independent | Fusobacterium varium | |
| 5.4.99.1 | 5'-deoxyadenosylcobalamin | i.e. coenzyme B12, required for activity | Fusobacterium varium | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 5.1.1.3 | Fusobacterium varium | the racemase is constitutive and not induced by D-glutamate | additional information |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.3.1.2 | physiological function | major role for the methylaspartate pathway of glutamate catabolism in Fusobacterium varium and substantial participation of the hydroxyglutarate pathway when coenzyme B12 is not available | Fusobacterium varium |
| 5.4.99.1 | metabolism | glutamate mutase and methylaspartase are the first two enzymes in the methylaspartate pathway, overview | Fusobacterium varium |
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