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Literature summary extracted from

  • Honda, Y.; Shimaya, N.; Ishisaki, K.; Ebihara, M.; Taniguchi, H.
    Elucidation of exo-beta-D-glucosaminidase activity of a family 9 glycoside hydrolase (PBPRA0520) from Photobacterium profundum SS9 (2011), Glycobiology, 21, 503-511.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.165 expressed in Escherichia coli BL21(DE3) cells Photobacterium profundum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.165 0.69
-
 4-nitrophenyl beta-D-glucosaminide pH 7.0, 37°C Photobacterium profundum
3.2.1.165 1.2
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum
3.2.1.165 1.4
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum
3.2.1.165 2.5
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum
3.2.1.165 4.5
-
 4-nitrophenyl beta-D-glucoside pH 7.0, 37°C Photobacterium profundum
3.2.1.165 4.7
-
 chitobiose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 17.5
-
 cellotriose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 18.8
-
 cellotetraose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 28.6
-
 cellobiose pH 7.0, 37°C Photobacterium profundum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.165 Photobacterium profundum
-
-
-
3.2.1.165 Photobacterium profundum SS9
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.165 Ni-NTA agarose column chromatography Photobacterium profundum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.165 0.005
-
 crude extract, using 4-nitrophenyl beta-D-glucoside as substrate, at pH 7.0, 37°C Photobacterium profundum
3.2.1.165 0.046
-
 purified enzyme, using 4-nitrophenyl beta-D-glucoside as substrate, at pH 7.0, 37°C Photobacterium profundum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.165 4-nitrophenyl beta-D-glucosaminide + H2O
-
 Photobacterium profundum 4-nitrophenol + beta-D-glucosamine
-
 ?
3.2.1.165 4-nitrophenyl beta-D-glucoside + H2O
-
 Photobacterium profundum 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.165 4-nitrophenyl beta-D-glucoside + H2O
-
 Photobacterium profundum SS9 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.165 cellobiose + H2O
-
 Photobacterium profundum ?
-
 ?
3.2.1.165 cellobiose + H2O
-
 Photobacterium profundum SS9 ?
-
 ?
3.2.1.165 cellopentaose + H2O
-
 Photobacterium profundum ?
-
 ?
3.2.1.165 cellopentaose + H2O
-
 Photobacterium profundum SS9 ?
-
 ?
3.2.1.165 cellotetraose + H2O
-
 Photobacterium profundum ?
-
 ?
3.2.1.165 cellotriose + H2O
-
 Photobacterium profundum ?
-
 ?
3.2.1.165 cellotriose + H2O
-
 Photobacterium profundum SS9 ?
-
 ?
3.2.1.165 chitobiose + H2O
-
 Photobacterium profundum D-glucosamine
-
 ?
3.2.1.165 deacetylated chitosan + H2O slight hydrolysis Photobacterium profundum ?
-
 ?
3.2.1.165 GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O
-
 Photobacterium profundum GlcN(beta1-4)GlcN + D-glucosamine
-
 ?
3.2.1.165 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O
-
 Photobacterium profundum GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine
-
 ?
3.2.1.165 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + H2O
-
 Photobacterium profundum GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN + D-glucosamine
-
 ?
3.2.1.165 additional information the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose Photobacterium profundum ?
-
 ?
3.2.1.165 additional information the enzyme fails to release a reducing sugar from avicel and carboxylmethyl cellulose Photobacterium profundum SS9 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.165 exo-beta-D-glucosaminidase
-
 Photobacterium profundum
3.2.1.165 PBPRA0520 protein
-
 Photobacterium profundum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.165 50
-
 in 50 mM phosphate buffer at pH 7.0 Photobacterium profundum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.165 0.087
-
 4-nitrophenyl beta-D-glucosaminide pH 7.0, 37°C Photobacterium profundum
3.2.1.165 0.19
-
 4-nitrophenyl beta-D-glucoside pH 7.0, 37°C Photobacterium profundum
3.2.1.165 47.7
-
 cellotriose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 52
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum
3.2.1.165 54.2
-
 cellotetraose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 68.8
-
 cellobiose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 77.4
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum
3.2.1.165 97.6
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum
3.2.1.165 152.9
-
 chitobiose pH 7.0, 37°C Photobacterium profundum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.165 7
-
 at 37°C Photobacterium profundum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.165 6 9
-
 Photobacterium profundum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.165 0.043
-
 4-nitrophenyl beta-D-glucoside pH 7.0, 37°C Photobacterium profundum
3.2.1.165 0.126
-
 4-nitrophenyl beta-D-glucosaminide pH 7.0, 37°C Photobacterium profundum
3.2.1.165 2.4
-
 cellobiose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 2.5
-
 cellopentaose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 2.7
-
 cellotriose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 2.9
-
 cellotetraose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 31.3
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum
3.2.1.165 32.6
-
 chitobiose pH 7.0, 37°C Photobacterium profundum
3.2.1.165 41.9
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum
3.2.1.165 69.2
-
 GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN(beta1-4)GlcN pH 7.0, 37°C Photobacterium profundum