Literature summary extracted from

Quaderer, R.; Omura, S.; Ikeda, H.; Cane, D.
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis (2006), J. Am. Chem. Soc., 128, 13036-13037.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.15.32	expressed in Escherichia coli BL21(DE3) cells as a C-terminal-His6-tag protein	Streptomyces avermitilis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14.15.32	0.00333	-	(+/-)-pentalenene	recombinant enzyme, in 50 mM phosphate buffer, 10% (v/v) glycerol, pH 7.4, at 22°C	Streptomyces avermitilis

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.15.32	Streptomyces avermitilis	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.14.15.32	Ni-NTA column chromatography	Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.15.32	(+/-)-pentalenene + reduced ferredoxin [iron-sulfur] cluster + H+ + O2	-	Streptomyces avermitilis	pentalen-13-al + oxidized ferredoxin [iron-sulfur] cluster + H2O	-	r

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.15.32	CYP183A1	-	Streptomyces avermitilis
1.14.15.32	PtlI	-	Streptomyces avermitilis
1.14.15.32	SAV2999	-	Streptomyces avermitilis

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.14.15.32	0.00838	-	(+/-)-pentalenene	recombinant enzyme, in 50 mM phosphate buffer, 10% (v/v) glycerol, pH 7.4, at 22°C	Streptomyces avermitilis

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.14.15.32	8	-	-	Streptomyces avermitilis

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.15.32	NADPH	-	Streptomyces avermitilis

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Release 2023.1 (January 2023)