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Literature summary extracted from

  • Nunn, C.E.; Johnsen, U.; Schoenheit, P.; Fuhrer, T.; Sauer, U.; Hough, D.W.; Danson, M.J.
    Metabolism of pentose sugars in the hyperthermophilic archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius (2010), J. Biol. Chem., 285, 33701-33709.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.26 expression in Escherichia coli Saccharolobus solfataricus
2.3.3.9 expression in Escherichia coli Saccharolobus solfataricus
4.1.3.1 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.26 0.1
-
 NADH pH 7.5, 70°C, recombinant enzyme and native enzyme from xylose-grown cell extract Saccharolobus solfataricus
1.1.1.26 5
-
 glyoxylate pH 7.5, 70°C, recombinant enzyme and native enzyme from xylose-grown cell extract Saccharolobus solfataricus
1.1.1.359 0.18
-
 D-xylose pH 8.0, 70°C, recombinant enzyme Saccharolobus solfataricus
1.1.1.359 0.18
-
 D-xylose pH 8.0, 70°C, native enzyme from xylose-grown cell extract Saccharolobus solfataricus
1.1.1.359 0.47
-
 L-arabinose pH 8.0, 70°C, native enzyme from xylose-grown cell extract Saccharolobus solfataricus
1.1.1.359 0.5
-
 L-arabinose pH 8.0, 70°C, recombinant enzyme Saccharolobus solfataricus
1.2.1.26 0.37
-
 glycolaldehyde pH 6.0, 70°C, native enzyme form xylose-grown cells Saccharolobus solfataricus
2.3.3.9 0.002
-
 glyoxylate pH 8.0, 70°C, recombinant enzyme Saccharolobus solfataricus
2.3.3.9 0.002
-
 glyoxylate pH 8.0, 70°C, native enzyme from xylose-grown cell extract Saccharolobus solfataricus
2.3.3.9 0.05
-
 acetyl-CoA pH 8.0, 70°C, native enzyme from xylose-grown cell extract Saccharolobus solfataricus
2.3.3.9 0.06
-
 acetyl-CoA pH 8.0, 70°C, recombinant enzyme Saccharolobus solfataricus
4.1.3.1 0.96
-
 isocitrate pH 7.5, 70°C, recombinant enzyme Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 189000
-
-
 Saccharolobus solfataricus
4.1.3.1 190000
-
-
 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.55 2-dehydro-3-deoxy-D-xylonate Saccharolobus solfataricus non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose pyruvate + glycolaldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-xylonate Saccharolobus solfataricus P2 non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose pyruvate + glycolaldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-L-arabinonate Saccharolobus solfataricus non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose pyruvate + glycolaldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-L-arabinonate Saccharolobus solfataricus P2 non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose pyruvate + glycolaldehyde
-
 ?
4.2.1.39 D-gluconate Saccharolobus solfataricus
-
 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.39 D-gluconate Saccharolobus solfataricus P2
-
 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.39 additional information Saccharolobus solfataricus gluconate dehydratase being active only with C6 metabolites ?
-
 ?
4.2.1.39 additional information Saccharolobus solfataricus P2 gluconate dehydratase being active only with C6 metabolites ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.26 Saccharolobus solfataricus Q97U35
-
-
1.1.1.26 Saccharolobus solfataricus P2 Q97U35
-
-
1.1.1.359 Saccharolobus solfataricus O93715
-
-
1.1.1.359 Saccharolobus solfataricus P2 O93715
-
-
1.2.1.26 Saccharolobus solfataricus Q97UA1
-
-
1.2.1.26 Saccharolobus solfataricus P2 Q97UA1
-
-
2.3.3.9 Saccharolobus solfataricus Q97YI7
-
-
2.3.3.9 Saccharolobus solfataricus P2 Q97YI7
-
-
4.1.2.55 Saccharolobus solfataricus O54288
-
-
4.1.2.55 Saccharolobus solfataricus P2 O54288
-
-
4.1.3.1 Saccharolobus solfataricus Q97YI8
-
-
4.1.3.1 Saccharolobus solfataricus P2 Q97YI8
-
-
4.2.1.39 Saccharolobus solfataricus
-
-
-
4.2.1.39 Saccharolobus solfataricus P2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.26
-
 Saccharolobus solfataricus
2.3.3.9
-
 Saccharolobus solfataricus
4.1.3.1
-
 Saccharolobus solfataricus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.26 culture condition:xylose-grown cell
-
 Saccharolobus solfataricus
-
1.1.1.359 culture condition:xylose-grown cell
-
 Saccharolobus solfataricus
-
1.2.1.26 culture condition:xylose-grown cell
-
 Saccharolobus solfataricus
-
2.3.3.9 culture condition:xylose-grown cell
-
 Saccharolobus solfataricus
-
4.1.3.1 culture condition:xylose-grown cell
-
 Saccharolobus solfataricus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.26 glyoxylate + NADH + H+
-
 Saccharolobus solfataricus glycolate + NAD+
-
 ?
1.1.1.26 glyoxylate + NADH + H+
-
 Saccharolobus solfataricus P2 glycolate + NAD+
-
 ?
1.1.1.359 D-xylose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 D-xylose + NADP+ + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
1.1.1.359 L-arabinose + NADP+ + H2O
-
 Saccharolobus solfataricus ?
-
 ?
1.1.1.359 L-arabinose + NADP+ + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
1.2.1.26 2,5-dioxopentanoate + NADP+ + H2O the enzyme is specific for NADP+ Saccharolobus solfataricus 2-oxoglutarate + NADPH + 2 H+
-
 ?
1.2.1.26 2,5-dioxopentanoate + NADP+ + H2O the enzyme is specific for NADP+ Saccharolobus solfataricus P2 2-oxoglutarate + NADPH + 2 H+
-
 ?
1.2.1.26 DL-glyceraldehyde + NADP+ + H2O the enzyme is specific for NADP+ Saccharolobus solfataricus glycerate + NADPH + H+
-
 ?
1.2.1.26 DL-glyceraldehyde + NADP+ + H2O the enzyme is specific for NADP+ Saccharolobus solfataricus P2 glycerate + NADPH + H+
-
 ?
1.2.1.26 glycolaldehyde + NADP+ + H2O the enzyme is specific for NADP+ Saccharolobus solfataricus glycolate + NADPH + H+
-
 ?
1.2.1.26 glycolaldehyde + NADP+ + H2O the enzyme is specific for NADP+ Saccharolobus solfataricus P2 glycolate + NADPH + H+
-
 ?
2.3.3.9 acetyl-CoA + H2O + glyoxylate
-
 Saccharolobus solfataricus (S)-malate + CoA
-
 ?
2.3.3.9 acetyl-CoA + H2O + glyoxylate
-
 Saccharolobus solfataricus P2 (S)-malate + CoA
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-xylonate non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose Saccharolobus solfataricus pyruvate + glycolaldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-xylonate since the substrate is not commercially available a coupled assay is carried out Saccharolobus solfataricus pyruvate + glycolaldehyde
-
 r
4.1.2.55 2-dehydro-3-deoxy-D-xylonate non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose Saccharolobus solfataricus P2 pyruvate + glycolaldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-D-xylonate since the substrate is not commercially available a coupled assay is carried out Saccharolobus solfataricus P2 pyruvate + glycolaldehyde
-
 r
4.1.2.55 2-dehydro-3-deoxy-L-arabinonate non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose Saccharolobus solfataricus pyruvate + glycolaldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-L-arabinonate since the substrate is not commercially available a coupled assay is carried out Saccharolobus solfataricus pyruvate + glycolaldehyde
-
 r
4.1.2.55 2-dehydro-3-deoxy-L-arabinonate non-phosphorylative Entner-Doudoroff pathway for catabolism of D-xylose and L-arabinose Saccharolobus solfataricus P2 pyruvate + glycolaldehyde
-
 ?
4.1.2.55 2-dehydro-3-deoxy-L-arabinonate since the substrate is not commercially available a coupled assay is carried out Saccharolobus solfataricus P2 pyruvate + glycolaldehyde
-
 r
4.1.2.55 pyruvate + glycolaldehyde
-
 Saccharolobus solfataricus 2-dehydro-3-deoxy-D-xylonate
-
 r
4.1.2.55 pyruvate + glycolaldehyde
-
 Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-xylonate
-
 r
4.1.2.55 pyruvate + glycolaldehyde
-
 Saccharolobus solfataricus 2-dehydro-3-deoxy-L-arabinonate
-
 r
4.1.3.1 isocitrate
-
 Saccharolobus solfataricus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Saccharolobus solfataricus P2 succinate + glyoxylate
-
 ?
4.2.1.39 D-gluconate
-
 Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.39 D-gluconate
-
 Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.39 additional information gluconate dehydratase being active only with C6 metabolites Saccharolobus solfataricus ?
-
 ?
4.2.1.39 additional information gluconate dehydratase being active only with C6 metabolites Saccharolobus solfataricus P2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.9 homodimer
-
 Saccharolobus solfataricus
4.1.3.1 homotetramer
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.26 SSO3187
-
 Saccharolobus solfataricus
1.2.1.26 SSO3117
-
 Saccharolobus solfataricus
2.3.3.9 SSO1334
-
 Saccharolobus solfataricus
4.1.2.55 KDG-aldolase
-
 Saccharolobus solfataricus
4.1.3.1 SSO1333
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.26 70
-
 assay at Saccharolobus solfataricus
1.1.1.359 70
-
 assay at Saccharolobus solfataricus
1.2.1.26 70
-
 assay at Saccharolobus solfataricus
2.3.3.9 70
-
 assay at Saccharolobus solfataricus
4.1.2.55 70
-
 assay at Saccharolobus solfataricus
4.1.3.1 70
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.26 7.5
-
 assay at Saccharolobus solfataricus
1.1.1.359 8
-
 assay at Saccharolobus solfataricus
1.2.1.26 6
-
 assay at Saccharolobus solfataricus
2.3.3.9 8
-
 assay at Saccharolobus solfataricus
4.1.2.55 6
-
 assay at Saccharolobus solfataricus
4.1.3.1 7.5
-
 assay at Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.26 NADH
-
 Saccharolobus solfataricus
1.1.1.359 NADP+
-
 Saccharolobus solfataricus
1.2.1.26 NADP+ the enzyme is specific for NADP+ Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
4.2.1.39 metabolism the enzyme is involved in the non-phosphorylative version of the Entner-Doudoroff pathway, overview. In vitro kinetic analyses of glucose dehydrogenase, gluconate dehydratase, and KDG-aldolase from Sulfolobus solfataricus show, that these enzymes are also capable of catalyzing the catabolism of galactose, the C4 epimer of glucose, to pyruvate and glyceraldehyde. Gluconate dehydratase is active only with C6 metabolites, thus it is not involved in the C5-specific branch of the non-phosphorylative version of the Entner-Doudoroff pathway Saccharolobus solfataricus