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Literature summary extracted from

  • Vester-Christensen, M.B.; Abou Hachem, M.; Svensson, B.; Henriksen, A.
    Crystal structure of an essential enzyme in seed starch degradation: barley limit dextrinase in complex with cyclodextrins (2010), J. Mol. Biol., 403, 739-750.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.142 expressed in Pichia pastoris Hordeum vulgare

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.142 in complex with the competitive inhibitor beta-cyclodextrin, hanging drop vapor diffusion method, with streak seeding in a reservoir solution of 22% (w/v) PEG 3350, 5% (v/v) Gol, and 0.3 M NaI, or without seeding in a reservoir solution of 30% (w/v) PEG 3350, 10% (v/v) Gol, and 0.3 M NaI. In complex with the competitive inhibitor alpha-cyclodextrin, hanging drop vapor diffusion method, without seeding in a reservoir solution of 30% (w/v) PEG 3350 and 0.3 M NaI Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.142 alpha-cyclodextrin competitive inhibitor Hordeum vulgare
3.2.1.142 beta-cyclodextrin competitive inhibitor Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.142 Ca2+ contains Ca2+ Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.142 Hordeum vulgare
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.142 limit dextrins + H2O limit dextrinase catalyzes the hydrolysis of alpha-1,6 glucosidic linkages in limit dextrins Hordeum vulgare ?
-
 ?
3.2.1.142 additional information the enzyme has low activity towards amylopectin Hordeum vulgare ?
-
 ?
3.2.1.142 pullulan + H2O
-
 Hordeum vulgare ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.142 78
-
 pullulan pH and temperature not specified in the publication Hordeum vulgare

General Information

EC Number General Information Comment Organism
3.2.1.142 physiological function limit dextrinase activity plays a role in starch degradation during germination and in starch biosynthesis during grain filling Hordeum vulgare