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Literature summary extracted from

  • McDevitt, C.; Hugenholtz, P.; Hanson, G.; McEwan, A.
    Molecular analysis of dimethyl sulphide dehydrogenase from Rhodovulum sulfidophilum: Its place in the dimethyl sulphoxide reductase family of microbial molybdopterin-containing enzymes (2002), Mol. Microbiol., 44, 1575-1587.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.2.4 periplasm
-
 Rhodovulum sulfidophilum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.4 Iron the enzyme contains 17 mol iron per mol enzyme Rhodovulum sulfidophilum
1.8.2.4 Molybdenum the enzyme contains 0.5 mol molybdenum per mol enzyme Rhodovulum sulfidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.4 32000
-
 subunit DdhC Rhodovulum sulfidophilum
1.8.2.4 32000
-
 1 * 94000 + 1 * 38000 + 1 * 32000 Rhodovulum sulfidophilum
1.8.2.4 38000
-
 subunit DdhB Rhodovulum sulfidophilum
1.8.2.4 38000
-
 1 * 94000 + 1 * 38000 + 1 * 32000 Rhodovulum sulfidophilum
1.8.2.4 94000
-
 subunit DdhA Rhodovulum sulfidophilum
1.8.2.4 94000
-
 1 * 94000 + 1 * 38000 + 1 * 32000 Rhodovulum sulfidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.2.4 dimethyl sulfide + ferricytochrome c2 + H2O Rhodovulum sulfidophilum
-
 dimethyl sulfoxide + ferrocytochrome c2
-
 ?
1.8.2.4 dimethyl sulfide + ferricytochrome c2 + H2O Rhodovulum sulfidophilum SH1
-
 dimethyl sulfoxide + ferrocytochrome c2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.4 Rhodovulum sulfidophilum Q8GPG1 subunit gamma (DdhC)
-
1.8.2.4 Rhodovulum sulfidophilum Q8GPG3 subunit beta (DdhB)
-
1.8.2.4 Rhodovulum sulfidophilum Q8GPG4 subunit alpha (DdhA)
-
1.8.2.4 Rhodovulum sulfidophilum SH1 Q8GPG1 subunit gamma (DdhC)
-
1.8.2.4 Rhodovulum sulfidophilum SH1 Q8GPG3 subunit beta (DdhB)
-
1.8.2.4 Rhodovulum sulfidophilum SH1 Q8GPG4 subunit alpha (DdhA)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.4 Poros 20HQ Strong anion exchange column chromatography and gel filtration Rhodovulum sulfidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.4 dimethyl sulfide + ferricytochrome c2 + H2O
-
 Rhodovulum sulfidophilum dimethyl sulfoxide + ferrocytochrome c2
-
 ?
1.8.2.4 dimethyl sulfide + ferricytochrome c2 + H2O
-
 Rhodovulum sulfidophilum SH1 dimethyl sulfoxide + ferrocytochrome c2
-
 ?
1.8.2.4 dimethyl sulfide + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Rhodovulum sulfidophilum dimethyl sulfoxide + reduced 2,6-dichlorophenolindophenol
-
 ?
1.8.2.4 dimethyl sulfide + oxidized 2,6-dichlorophenolindophenol + H2O
-
 Rhodovulum sulfidophilum SH1 dimethyl sulfoxide + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.4 heterotrimer 1 * 94000 + 1 * 38000 + 1 * 32000 Rhodovulum sulfidophilum

Synonyms

EC Number Synonyms Comment Organism
1.8.2.4 DdhA subunit Rhodovulum sulfidophilum
1.8.2.4 DdhB subunit Rhodovulum sulfidophilum
1.8.2.4 DdhC subunit Rhodovulum sulfidophilum
1.8.2.4 dimethyl sulphide dehydrogenase
-
 Rhodovulum sulfidophilum
1.8.2.4 DMS dehydrogenase
-
 Rhodovulum sulfidophilum
1.8.2.4 DMS DH
-
 Rhodovulum sulfidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.4 molybdopterin bis(molybdopterin guanine dinucleotide)Mo Rhodovulum sulfidophilum
1.8.2.4 [3Fe-4S]-center subunit DdhB binds one 3Fe-4S cluster Rhodovulum sulfidophilum

General Information

EC Number General Information Comment Organism
1.8.2.4 malfunction the ddhA mutant is not able to grow photoautotrophically with dimethyl sulfide as electron donor, although photoheterotrophic growth is not impaired Rhodovulum sulfidophilum
1.8.2.4 physiological function dimethyl sulfide dehydrogenase is essential for photolithotrophic growth of Rhodovulum sulfidophilum on dimethyl sulfide but not for chemo-trophic growth on the same substrate Rhodovulum sulfidophilum