EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.99.17 | LeThiC under the control of the cauliflower mosaic virus 35S promoter is introduced into the tl mutant by Agrobacterium tumefaciens-mediated transformation. Expression of the wild-type LeThiC gene in the tl mutant is able to complement the mutant to wild type | Solanum lycopersicum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.99.17 | chloroplast | - |
Solanum lycopersicum | 9507 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.17 | iron-sulfur centre | - |
Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | Solanum lycopersicum | the enzyme is involved in thiamin biosynthesis | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.17 | Solanum lycopersicum | B9TU32 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.99.17 | leaf | - |
Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | - |
Solanum lycopersicum | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? | |
4.1.99.17 | 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine | the enzyme is involved in thiamin biosynthesis | Solanum lycopersicum | 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.17 | LeThiC | - |
Solanum lycopersicum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.1.99.17 | Solanum lycopersicum | expression of LeThiC is tightly regulated at the transcriptional and posttranscriptional level by multiple factors, such as light, Fe2+ status and thiamine diphosphate-riboswitch. A feedback regulation mechanism is involved in synthesis of the pyrimidine moiety for controlling thiamine synthesis in tomato | additional information |
4.1.99.17 | Solanum lycopersicum | the abundance of LeTHIC expression is dependent on light | up |
4.1.99.17 | Solanum lycopersicum | the expression intensity of LeThic at both the transcriptional (S form mRNA) and protein levels is increased under Fe2+ deficiency (1 or 0 mM Fe2+) compared with Fe2+ sufficiency (10 and 100 mM Fe2+), whereas no effects on LeThiC expression are observed under deficiency of Zn2+ or Mn2+ | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.99.17 | physiological function | the enzyme is involved in thiamine biosynthesis | Solanum lycopersicum |