EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.4 | Ca2+ | interacts with the SDS contained in the mixed micelles thus leading to an aggregated form of the substrate which is more easily hydrolyzed by PLDalpha | Helianthus annuus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.4 | additional information | Helianthus annuus | purified PLDalpha is inactive in vitro on bilamellar substrates. It is fully active on mixed micelles made with phospholipids and a mixture of Triton-X100 and SDS at equal concentrations. Ca2+ interacts with the SDS contained in the mixed micelles thus leading to an aggregated form of the substrate which is more easily hydrolyzed by PLDalpha | ? | - |
? | |
3.1.4.4 | phosphatidylcholine + H2O | Helianthus annuus | - |
choline + phosphatidate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.4 | Helianthus annuus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.4 | native PLDalpha 25fold from six-day post-germinated seeds | Helianthus annuus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.4 | seed | - |
Helianthus annuus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.4 | 60 | - |
purified native enzyme, pH and temperature not specified in the publication | Helianthus annuus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.4 | additional information | purified PLDalpha is inactive in vitro on bilamellar substrates. It is fully active on mixed micelles made with phospholipids and a mixture of Triton-X100 and SDS at equal concentrations. Ca2+ interacts with the SDS contained in the mixed micelles thus leading to an aggregated form of the substrate which is more easily hydrolyzed by PLDalpha | Helianthus annuus | ? | - |
? | |
3.1.4.4 | phosphatidylcholine + H2O | - |
Helianthus annuus | choline + phosphatidate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.4 | phospholipase Dalpha | - |
Helianthus annuus |
3.1.4.4 | PLDalpha | - |
Helianthus annuus |