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Literature summary extracted from

  • Zhang, Q.; Shirley, N.J.; Burton, R.A.; Lahnstein, J.; Hrmova, M.; Fincher, G.B.
    The genetics, transcriptional profiles, and catalytic properties of UDP-alpha-D-xylose 4-epimerases from barley (2010), Plant Physiol., 153, 555-568.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.5 dithiothreitol
-
 Hordeum vulgare
5.1.3.5 dithiothreitol 150% activity at 2 mM Hordeum vulgare

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.5 isoform UXE1 is expressed in Pichia pastoris Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.5 additional information UMP and EDTA do not inhibit the activity of the recombinant isoform UXE1 Hordeum vulgare
5.1.3.5 UDP 15% residual activity at 2 mM Hordeum vulgare
5.1.3.5 UTP 54% residual activity at 2 mM Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.5 1.4
-
 UDP-beta-L-arabinopyranose pH 7.0, at 25°C Hordeum vulgare
5.1.3.5 1.8
-
 UDP-alpha-D-xylopyranose pH 7.0, at 25°C Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.1.3.5 additional information Mg2+ does not stimulate the activity of the recombinant isoform UXE1. Addition of NADH or NAD+ has no significant effect on activity Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.5 45000
-
 isoform UXE3, calculated from amino acid sequence Hordeum vulgare
5.1.3.5 47000
-
 isoform UXE1, calculated from amino acid sequence Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.5 Hordeum vulgare Q2LC81 isoform UXE3
-
5.1.3.5 Hordeum vulgare Q2LC82 isoform UXE2
-
5.1.3.5 Hordeum vulgare Q2LC83 isoform UXE1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.5 Ni-NTA column chromatography Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.5 endosperm
-
 Hordeum vulgare
-

Storage Stability

EC Number Storage Stability Organism
5.1.3.5 4°C, purified enzyme, at least 1 week, little or no loss of activity Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.5 additional information the purified recombinant isoform UXE1 does not catalyze the interconversion of UDP-glucose and UDP-galactose Hordeum vulgare ?
-
 ?
5.1.3.5 UDP-alpha-D-xylopyranose
-
 Hordeum vulgare UDP-beta-L-arabinopyranose
-
 r
5.1.3.5 UDP-beta-L-arabinopyranose
-
 Hordeum vulgare UDP-alpha-D-xylopyranose
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.5 UDP-alpha-D-xylose 4-epimerase
-
 Hordeum vulgare
5.1.3.5 UXE1 isoform Hordeum vulgare
5.1.3.5 UXE2 isoform Hordeum vulgare
5.1.3.5 UXE3 isoform Hordeum vulgare

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.1.3.5 1.5
-
 UDP-beta-L-arabinopyranose pH 7.0, at 25°C Hordeum vulgare
5.1.3.5 1.5
-
 UDP-alpha-D-xylopyranose pH 7.0, at 25°C Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.5 7
-
 about pH 7.0 Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.5 NAD+
-
 Hordeum vulgare
5.1.3.5 NAD+ each molecule of isoform UXE1 contains about one molecule of noncovalently bound NAD+ Hordeum vulgare

Expression

EC Number Organism Comment Expression
5.1.3.5 Hordeum vulgare isoform UXE2 transcript abundance is moderate during the syncytial stage of endosperm development (2-6 days after pollination) but decreases to undetectable levels beyond 6 days after pollination down
5.1.3.5 Hordeum vulgare in developing barley endosperm, isoform UXE3 mRNA levels peak at a time when UDP-alpha-D-xylopyranose synthase transcripts also reach a maximum and when arabinoxylan biosynthesis is initiated up
5.1.3.5 Hordeum vulgare in developing barley endosperm, isoforms UXE1 mRNA levels peak at a time when UDP-alpha-D-xylopyranose synthase transcripts also reach a maximum and when arabinoxylan biosynthesis is initiated. Transcript levels for the UXE1 gene are about 4fold higher at 9 days after pollination than in the syncytial and cellularization stages up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.1.3.5 0.9
-
 UDP-alpha-D-xylopyranose pH 7.0, at 25°C Hordeum vulgare
5.1.3.5 1.1
-
 UDP-beta-L-arabinopyranose pH 7.0, at 25°C Hordeum vulgare