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Literature summary extracted from

  • Dionisio, G.; Madsen, C.; Holm, P.; Welinder, K.; Jorgensen, M.; Stoger, E.; Arcalis, E.; Brinch-Pedersen, H.
    Cloning and characterization of purple acid phosphatase phytases from wheat, barley, maize, and rice (2011), Plant Physiol., 156, 1087-1100.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.8 phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium Triticum aestivum
3.1.3.8 phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium Hordeum vulgare
3.1.3.8 phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium Zea mays
3.1.3.26 phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 additional information
-
additional information substrate specificity and kinetics of recombinant isozymes, overview Triticum aestivum
3.1.3.8 additional information
-
additional information substrate specificity and kinetics of recombinant isozymes, overview Hordeum vulgare
3.1.3.8 additional information
-
additional information substrate specificity and kinetics of recombinant isozymes, overview Zea mays
3.1.3.8 0.035
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a1, pH 5.0, 36°C Triticum aestivum
3.1.3.8 0.036
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a, pH 5.0, 36°C Hordeum vulgare
3.1.3.8 0.045
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b1, pH 5.0, 36°C Triticum aestivum
3.1.3.8 0.046
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b2, pH 5.0, 36°C Hordeum vulgare
3.1.3.8 0.048
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Zea mays
3.1.3.26 additional information
-
additional information substrate specificity and kinetics of recombinant isozymes, overview Oryza sativa Japonica Group
3.1.3.26 0.054
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.8 Fe2+ activates isozyme b1 about 5fold at 10 mM Triticum aestivum
3.1.3.8 Mn2+ activates isozyme a1 12fold at 10 mM Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 66000
-
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Triticum aestivum
3.1.3.8 66000
-
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Hordeum vulgare
3.1.3.8 66000
-
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Zea mays
3.1.3.26 66000
-
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Triticum aestivum
-
? + phosphate
-
?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Hordeum vulgare
-
? + phosphate
-
?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Zea mays
-
? + phosphate
-
?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Triticum aestivum Bob white SH 98 26
-
? + phosphate
-
?
3.1.3.26 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Oryza sativa Japonica Group
-
? + phosphate
-
?
3.1.3.26 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O Oryza sativa Japonica Group Himalaya
-
? + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Hordeum vulgare C4PKL2 isozyme a; Golden Promise, isozyme a
-
3.1.3.8 Hordeum vulgare C4PKL4 isozyme b2; Golden Promise, isozyme b2
-
3.1.3.8 Triticum aestivum C4PKK7 isozyme a1; isozyme a1
-
3.1.3.8 Triticum aestivum C4PKK9 isozyme b1; isozyme b1
-
3.1.3.8 Triticum aestivum Bob white SH 98 26 C4PKK7 isozyme a1; isozyme a1
-
3.1.3.8 Triticum aestivum Bob white SH 98 26 C4PKK9 isozyme b1; isozyme b1
-
3.1.3.8 Zea mays C4PKL6 isozyme b; isozyme b
-
3.1.3.26 Oryza sativa Japonica Group D6QSX9 isozyme b; isozyme b
-
3.1.3.26 Oryza sativa Japonica Group Himalaya D6QSX9 isozyme b; isozyme b
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.8 glycoprotein the monomeric enzyme is glycosylated to different degrees, the isozyme contains 7 N-glycosylation sites Zea mays
3.1.3.8 glycoprotein the monomeric enzyme is glycosylated to different degrees, the isozyme contains 8 N-glycosylation sites Hordeum vulgare
3.1.3.8 glycoprotein the monomeric enzyme is glycosylated to different degrees, the isozyme contains 9 N-glycosylation sites Triticum aestivum
3.1.3.26 glycoprotein the monomeric enzyme is glycosylated to different degrees, the isozyme contains 8 N-glycosylation sites Oryza sativa Japonica Group

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 soluble screted recombinant enzyme from Pichia pastoris culture supernatant by gel filtration Zea mays
3.1.3.8 soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration Triticum aestivum
3.1.3.8 soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration Hordeum vulgare
3.1.3.26 soluble screted recombinant enzyme from Pichia pastoris culture supernatant by gel filtration Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.8 additional information identification of wheat phytase in mature and germinating grains by tandem mass spectrometry analysis Triticum aestivum
-
3.1.3.8 seed
-
Zea mays
-
3.1.3.8 seed quantitative reverse transcription PCR enzyme expression analysis in developing grains Triticum aestivum
-
3.1.3.8 seed quantitative reverse transcription PCR enzyme expression analysis in developing grains Hordeum vulgare
-
3.1.3.26 seed
-
Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 additional information substrate specificity of recombinant isozymes, overview Triticum aestivum ?
-
?
3.1.3.8 additional information substrate specificity of recombinant isozymes, overview Hordeum vulgare ?
-
?
3.1.3.8 additional information substrate specificity of the recombinant isozyme, overview Zea mays ?
-
?
3.1.3.8 additional information substrate specificity of recombinant isozymes, overview Triticum aestivum Bob white SH 98 26 ?
-
?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Triticum aestivum ? + phosphate
-
?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Hordeum vulgare ? + phosphate
-
?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Zea mays ? + phosphate
-
?
3.1.3.8 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Triticum aestivum Bob white SH 98 26 ? + phosphate
-
?
3.1.3.26 additional information substrate specificity of the recombinant isozyme, overview Oryza sativa Japonica Group ?
-
?
3.1.3.26 additional information substrate specificity of the recombinant isozyme, overview Oryza sativa Japonica Group Himalaya ?
-
?
3.1.3.26 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Oryza sativa Japonica Group ? + phosphate
-
?
3.1.3.26 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
Oryza sativa Japonica Group Himalaya ? + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Triticum aestivum
3.1.3.8 monomer 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Hordeum vulgare
3.1.3.8 monomer 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Zea mays
3.1.3.26 monomer 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 PAP
-
Triticum aestivum
3.1.3.8 PAP
-
Hordeum vulgare
3.1.3.8 PAP
-
Zea mays
3.1.3.8 PAP type I
-
Triticum aestivum
3.1.3.8 PAP type I
-
Hordeum vulgare
3.1.3.8 PAP type I
-
Zea mays
3.1.3.8 PAPhys
-
Triticum aestivum
3.1.3.8 PAPhys
-
Hordeum vulgare
3.1.3.8 PAPhys
-
Zea mays
3.1.3.8 purple acid phosphatase
-
Triticum aestivum
3.1.3.8 purple acid phosphatase
-
Hordeum vulgare
3.1.3.8 purple acid phosphatase
-
Zea mays
3.1.3.26 PAP
-
Oryza sativa Japonica Group
3.1.3.26 PAP type I
-
Oryza sativa Japonica Group
3.1.3.26 PAPhys
-
Oryza sativa Japonica Group
3.1.3.26 purple acid phosphatase
-
Oryza sativa Japonica Group

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 36
-
assay at Triticum aestivum
3.1.3.8 36
-
assay at Hordeum vulgare
3.1.3.8 36
-
assay at Zea mays
3.1.3.26 36
-
assay at Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 248
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Zea mays
3.1.3.8 253
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b2, pH 5.0, 36°C Hordeum vulgare
3.1.3.8 260
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a, pH 5.0, 36°C Hordeum vulgare
3.1.3.8 270
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b1, pH 5.0, 36°C Triticum aestivum
3.1.3.8 279
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a1, pH 5.0, 36°C Triticum aestivum
3.1.3.26 231
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
assay at Triticum aestivum
3.1.3.8 5
-
assay at Hordeum vulgare
3.1.3.8 5
-
assay at Zea mays
3.1.3.26 5
-
assay at Oryza sativa Japonica Group

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.8 517
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Zea mays
3.1.3.8 550
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b2, pH 5.0, 36°C Hordeum vulgare
3.1.3.8 600
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b1, pH 5.0, 36°C Triticum aestivum
3.1.3.8 722
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a, pH 5.0, 36°C Hordeum vulgare
3.1.3.8 796
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme a1, pH 5.0, 36°C Triticum aestivum
3.1.3.26 428
-
myo-inositol-1,2,3,4,5,6-hexakisphosphate recombinant isozyme b, pH 5.0, 36°C Oryza sativa Japonica Group