EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium | Triticum aestivum |
3.1.3.8 | phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium | Hordeum vulgare |
3.1.3.8 | phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium | Zea mays |
3.1.3.26 | phylogenetic tree, functional enzyme expression in Pichia pastoris. The Pichia pastoris PHO1 phosphatase is repressed by 0.1 M phosphate buffer, and nontransformed or empty vector-transformed Pichia pastoris shows no detectable secretion or cell wall-associated phosphatase or phytase activity during 5 d of culture. Predicted endoplasmic reticulum signal peptides and potential C-terminal membrane retention signals are excised from the expression constructs, the recombinant enzyme is secreted to the culture medium | Oryza sativa Japonica Group |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | additional information | - |
additional information | substrate specificity and kinetics of recombinant isozymes, overview | Triticum aestivum | |
3.1.3.8 | additional information | - |
additional information | substrate specificity and kinetics of recombinant isozymes, overview | Hordeum vulgare | |
3.1.3.8 | additional information | - |
additional information | substrate specificity and kinetics of recombinant isozymes, overview | Zea mays | |
3.1.3.8 | 0.035 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a1, pH 5.0, 36°C | Triticum aestivum | |
3.1.3.8 | 0.036 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a, pH 5.0, 36°C | Hordeum vulgare | |
3.1.3.8 | 0.045 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b1, pH 5.0, 36°C | Triticum aestivum | |
3.1.3.8 | 0.046 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b2, pH 5.0, 36°C | Hordeum vulgare | |
3.1.3.8 | 0.048 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Zea mays | |
3.1.3.26 | additional information | - |
additional information | substrate specificity and kinetics of recombinant isozymes, overview | Oryza sativa Japonica Group | |
3.1.3.26 | 0.054 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Oryza sativa Japonica Group |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Fe2+ | activates isozyme b1 about 5fold at 10 mM | Triticum aestivum | |
3.1.3.8 | Mn2+ | activates isozyme a1 12fold at 10 mM | Triticum aestivum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 66000 | - |
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Triticum aestivum |
3.1.3.8 | 66000 | - |
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Hordeum vulgare |
3.1.3.8 | 66000 | - |
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Zea mays |
3.1.3.26 | 66000 | - |
1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Oryza sativa Japonica Group |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Triticum aestivum | - |
? + phosphate | - |
? | |
3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Hordeum vulgare | - |
? + phosphate | - |
? | |
3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Zea mays | - |
? + phosphate | - |
? | |
3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Triticum aestivum Bob white SH 98 26 | - |
? + phosphate | - |
? | |
3.1.3.26 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Oryza sativa Japonica Group | - |
? + phosphate | - |
? | |
3.1.3.26 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | Oryza sativa Japonica Group Himalaya | - |
? + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Hordeum vulgare | C4PKL2 | isozyme a; Golden Promise, isozyme a | - |
3.1.3.8 | Hordeum vulgare | C4PKL4 | isozyme b2; Golden Promise, isozyme b2 | - |
3.1.3.8 | Triticum aestivum | C4PKK7 | isozyme a1; isozyme a1 | - |
3.1.3.8 | Triticum aestivum | C4PKK9 | isozyme b1; isozyme b1 | - |
3.1.3.8 | Triticum aestivum Bob white SH 98 26 | C4PKK7 | isozyme a1; isozyme a1 | - |
3.1.3.8 | Triticum aestivum Bob white SH 98 26 | C4PKK9 | isozyme b1; isozyme b1 | - |
3.1.3.8 | Zea mays | C4PKL6 | isozyme b; isozyme b | - |
3.1.3.26 | Oryza sativa Japonica Group | D6QSX9 | isozyme b; isozyme b | - |
3.1.3.26 | Oryza sativa Japonica Group Himalaya | D6QSX9 | isozyme b; isozyme b | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.3.8 | glycoprotein | the monomeric enzyme is glycosylated to different degrees, the isozyme contains 7 N-glycosylation sites | Zea mays |
3.1.3.8 | glycoprotein | the monomeric enzyme is glycosylated to different degrees, the isozyme contains 8 N-glycosylation sites | Hordeum vulgare |
3.1.3.8 | glycoprotein | the monomeric enzyme is glycosylated to different degrees, the isozyme contains 9 N-glycosylation sites | Triticum aestivum |
3.1.3.26 | glycoprotein | the monomeric enzyme is glycosylated to different degrees, the isozyme contains 8 N-glycosylation sites | Oryza sativa Japonica Group |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | soluble screted recombinant enzyme from Pichia pastoris culture supernatant by gel filtration | Zea mays |
3.1.3.8 | soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration | Triticum aestivum |
3.1.3.8 | soluble screted recombinant isozyme from Pichia pastoris culture supernatant by gel filtration | Hordeum vulgare |
3.1.3.26 | soluble screted recombinant enzyme from Pichia pastoris culture supernatant by gel filtration | Oryza sativa Japonica Group |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.8 | additional information | identification of wheat phytase in mature and germinating grains by tandem mass spectrometry analysis | Triticum aestivum | - |
3.1.3.8 | seed | - |
Zea mays | - |
3.1.3.8 | seed | quantitative reverse transcription PCR enzyme expression analysis in developing grains | Triticum aestivum | - |
3.1.3.8 | seed | quantitative reverse transcription PCR enzyme expression analysis in developing grains | Hordeum vulgare | - |
3.1.3.26 | seed | - |
Oryza sativa Japonica Group | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | additional information | substrate specificity of recombinant isozymes, overview | Triticum aestivum | ? | - |
? | |
3.1.3.8 | additional information | substrate specificity of recombinant isozymes, overview | Hordeum vulgare | ? | - |
? | |
3.1.3.8 | additional information | substrate specificity of the recombinant isozyme, overview | Zea mays | ? | - |
? | |
3.1.3.8 | additional information | substrate specificity of recombinant isozymes, overview | Triticum aestivum Bob white SH 98 26 | ? | - |
? | |
3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Triticum aestivum | ? + phosphate | - |
? | |
3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Hordeum vulgare | ? + phosphate | - |
? | |
3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Zea mays | ? + phosphate | - |
? | |
3.1.3.8 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Triticum aestivum Bob white SH 98 26 | ? + phosphate | - |
? | |
3.1.3.26 | additional information | substrate specificity of the recombinant isozyme, overview | Oryza sativa Japonica Group | ? | - |
? | |
3.1.3.26 | additional information | substrate specificity of the recombinant isozyme, overview | Oryza sativa Japonica Group Himalaya | ? | - |
? | |
3.1.3.26 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Oryza sativa Japonica Group | ? + phosphate | - |
? | |
3.1.3.26 | myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O | - |
Oryza sativa Japonica Group Himalaya | ? + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.8 | monomer | 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Triticum aestivum |
3.1.3.8 | monomer | 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Hordeum vulgare |
3.1.3.8 | monomer | 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Zea mays |
3.1.3.26 | monomer | 1 * 66000, deglycosylated recombinant enzyme, SDS-PAGE | Oryza sativa Japonica Group |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.8 | PAP | - |
Triticum aestivum |
3.1.3.8 | PAP | - |
Hordeum vulgare |
3.1.3.8 | PAP | - |
Zea mays |
3.1.3.8 | PAP type I | - |
Triticum aestivum |
3.1.3.8 | PAP type I | - |
Hordeum vulgare |
3.1.3.8 | PAP type I | - |
Zea mays |
3.1.3.8 | PAPhys | - |
Triticum aestivum |
3.1.3.8 | PAPhys | - |
Hordeum vulgare |
3.1.3.8 | PAPhys | - |
Zea mays |
3.1.3.8 | purple acid phosphatase | - |
Triticum aestivum |
3.1.3.8 | purple acid phosphatase | - |
Hordeum vulgare |
3.1.3.8 | purple acid phosphatase | - |
Zea mays |
3.1.3.26 | PAP | - |
Oryza sativa Japonica Group |
3.1.3.26 | PAP type I | - |
Oryza sativa Japonica Group |
3.1.3.26 | PAPhys | - |
Oryza sativa Japonica Group |
3.1.3.26 | purple acid phosphatase | - |
Oryza sativa Japonica Group |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 36 | - |
assay at | Triticum aestivum |
3.1.3.8 | 36 | - |
assay at | Hordeum vulgare |
3.1.3.8 | 36 | - |
assay at | Zea mays |
3.1.3.26 | 36 | - |
assay at | Oryza sativa Japonica Group |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 248 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Zea mays | |
3.1.3.8 | 253 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b2, pH 5.0, 36°C | Hordeum vulgare | |
3.1.3.8 | 260 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a, pH 5.0, 36°C | Hordeum vulgare | |
3.1.3.8 | 270 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b1, pH 5.0, 36°C | Triticum aestivum | |
3.1.3.8 | 279 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a1, pH 5.0, 36°C | Triticum aestivum | |
3.1.3.26 | 231 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Oryza sativa Japonica Group |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 5 | - |
assay at | Triticum aestivum |
3.1.3.8 | 5 | - |
assay at | Hordeum vulgare |
3.1.3.8 | 5 | - |
assay at | Zea mays |
3.1.3.26 | 5 | - |
assay at | Oryza sativa Japonica Group |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 517 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Zea mays | |
3.1.3.8 | 550 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b2, pH 5.0, 36°C | Hordeum vulgare | |
3.1.3.8 | 600 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b1, pH 5.0, 36°C | Triticum aestivum | |
3.1.3.8 | 722 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a, pH 5.0, 36°C | Hordeum vulgare | |
3.1.3.8 | 796 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme a1, pH 5.0, 36°C | Triticum aestivum | |
3.1.3.26 | 428 | - |
myo-inositol-1,2,3,4,5,6-hexakisphosphate | recombinant isozyme b, pH 5.0, 36°C | Oryza sativa Japonica Group |