EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.18.1.2 | analysis of the enzyme in complex with a synthetic peptide representing the first FNR-binding repeat from Pisum sativum Tic62, including the strictly conserved core motif KTEQPLSPYTAYDDLKPPSSPSPTKPS, based on the crystal structure PDB ID 1QG0, X-ray diffraction structure analysis at 1.7 A resolution, molecular replacement | Pisum sativum |
EC Number | General Stability | Organism |
---|---|---|
1.18.1.2 | interaction with Tic62 stabilizes FNR | Pisum sativum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.18.1.2 | chloroplast | FNR is a soluble protein associated with thylakoid membranes | Pisum sativum | 9507 | - |
1.18.1.2 | thylakoid membrane | FNR is a soluble protein associated with thylakoid membranes | Pisum sativum | 42651 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | 2 reduced ferredoxin + NADP+ | Pisum sativum | - |
2 oxidized ferredoxin + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.2 | Pisum sativum | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | 2 reduced ferredoxin + NADP+ | - |
Pisum sativum | 2 oxidized ferredoxin + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.18.1.2 | More | FNR-membrane recruitment motif, FNR-MRM, forms a polyproline helix that interacts with a proline recognition domain on FNR. FNR displays a pH-dependent high affinity to the MRM, loop residues contribute to multiple interactions with both FNR subunits | Pisum sativum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.2 | ferredoxin:NADPH oxidoreductase | - |
Pisum sativum |
1.18.1.2 | FNR | - |
Pisum sativum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 6 | 8 | assay at | Pisum sativum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.18.1.2 | additional information | FNR interacts with Tic62, a member of the Tic complex, i.e. translocon at the inner envelope of chloroplasts, involved in redox-regulation of protein import into chloroplasts. Tic62 represents a major FNR interaction protein partner at the thylakoids, and binding to Tic62 clearly increases the stability of FNR. The specific interaction with FNR is mediated by a conserved sequence motif rich in proline and serine residues, located in the C terminus of Tic62 | Pisum sativum |