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Literature summary extracted from

  • Lei, C.; Rider, S.D.; Wang, C.; Zhang, H.; Tan, X.; Zhu, G.
    The apicomplexan Cryptosporidium parvum possesses a single mitochondrial-type ferredoxin and ferredoxin:NADP+ reductase system (2010), Protein Sci., 19, 2073-2084.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 mtFNR, sequence comparison and phylogenetic analysis, real-time quantitative RT-PCR expression ananlysis, recombinant expression of the His-tagged enzyme in Escherichia coli strain Rosetta (DE3) Cryptosporidium parvum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 additional information
-
 additional information Michaelis-Menten kinetics, overview Cryptosporidium parvum
1.18.1.2 0.0037
-
 NADPH pH 7.4, temperature not specified in the publication, recombinant enzyme Cryptosporidium parvum
1.18.1.2 0.0094
-
 NADPH pH 8.2, temperature not specified in the publication, recombinant enzyme Cryptosporidium parvum
1.18.1.2 0.0242
-
 NADPH pH 6.8, temperature not specified in the publication, recombinant enzyme Cryptosporidium parvum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 mitochondrion mtFNR Cryptosporidium parvum 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 2 reduced ferredoxin + NADP+ Cryptosporidium parvum
-
 2 oxidized ferredoxin + NADPH + H+
-
 r
1.18.1.2 2 reduced ferredoxin + NADP+ Cryptosporidium parvum IOWA-1
-
 2 oxidized ferredoxin + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Cryptosporidium parvum
-
 single copy FNR
-
1.18.1.2 Cryptosporidium parvum IOWA-1
-
 single copy FNR
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 recombinant His-tagged mtFNR to homogeneity from Escherichia coli by nickel affinity chromatography Cryptosporidium parvum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.18.1.2 additional information CpmtFd and CpmtFNR genes are constitutively transcribed during the complex parasite life cycle, real-time quantitative RT-PCR expression analysis, overview Cryptosporidium parvum
-
1.18.1.2 sporozoite
-
 Cryptosporidium parvum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 2 reduced ferredoxin + NADP+
-
 Cryptosporidium parvum 2 oxidized ferredoxin + NADPH + H+
-
 r
1.18.1.2 2 reduced ferredoxin + NADP+ cyt c-coupled assay, electron transfer system, overview Cryptosporidium parvum 2 oxidized ferredoxin + NADPH + H+
-
 r
1.18.1.2 2 reduced ferredoxin + NADP+
-
 Cryptosporidium parvum IOWA-1 2 oxidized ferredoxin + NADPH + H+
-
 r
1.18.1.2 2 reduced ferredoxin + NADP+ cyt c-coupled assay, electron transfer system, overview Cryptosporidium parvum IOWA-1 2 oxidized ferredoxin + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 More the mtFNR enzymatic region contains at least six highly conserved domains or motifs, including three FAD-diphosphate binding and one NADP+-diphosphate binding motifs Cryptosporidium parvum

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin:NADP+ reductase
-
 Cryptosporidium parvum
1.18.1.2 mitochondrial-type ferredoxin:NADP+ reductase
-
 Cryptosporidium parvum
1.18.1.2 mtFNR
-
 Cryptosporidium parvum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.2 25
-
 assay at Cryptosporidium parvum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 0.24
-
 NADPH pH 7.4, temperature not specified in the publication, recombinant enzyme Cryptosporidium parvum
1.18.1.2 0.6
-
 NADPH pH 8.2, temperature not specified in the publication, recombinant enzyme Cryptosporidium parvum
1.18.1.2 21.71
-
 NADPH pH 6.8, temperature not specified in the publication, recombinant enzyme Cryptosporidium parvum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 6.8
-
-
 Cryptosporidium parvum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.18.1.2 6.8 8.2 assay range Cryptosporidium parvum

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD
-
 Cryptosporidium parvum

General Information

EC Number General Information Comment Organism
1.18.1.2 physiological function in the photosystem I, an oxidized ferredoxin molecule, Fdox, first receives an electron driven by light energy to form a reduced ferredoxin, Fdred. The FAD-containing FNR then catalyzes the transfer of the electron to NADP+, which recycles Fdred back to Fdox. On the other hand, FNR mainly catalyzes the conversion from Fdox to Fdred in reactions other than photosynthesis Cryptosporidium parvum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 0.000000064
-
 NADPH pH 7.4, 25°C, recombinant enzyme Cryptosporidium parvum
1.18.1.2 0.000000064
-
 NADPH pH 8.2, 25°C, recombinant enzyme Cryptosporidium parvum
1.18.1.2 0.0000009
-
 NADPH pH 6.8, 25°C, recombinant enzyme Cryptosporidium parvum