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Literature summary extracted from

  • Phillips, G.; Swairjo, M.A.; Gaston, K.W.; Bailly, M.; Limbach, P.A.; Iwata-Reuyl, D.; de Crecy-Lagard, V.
    Diversity of archaeosine synthesis in crenarchaeota (2012), ACS Chem. Biol., 7, 300-305.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.97 in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulted deletion strain is transformed with an expression plasmid containing GAT-queC from S. solfataricus (SSO0016) cloned behind a PBAD promoter Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O Sulfurisphaera tokodaii
-
 L-glutamate + archaeine15 in tRNA i.e. G+, whcih is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O Euryarchaeota
-
 L-glutamate + archaeine15 in tRNA i.e. G+, which is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.97 Euryarchaeota
-
 overview, gene arcS
-
2.6.1.97 no activity in Pyrobaculum calidifontis
-
 strain JCM 11548
-
2.6.1.97 no activity in Pyrobaculum islandicum
-
-
-
2.6.1.97 no activity in Sulfolobus acidocaldarius
-
-
-
2.6.1.97 Saccharolobus solfataricus Q981C9
-
-
2.6.1.97 Saccharolobus solfataricus P2 Q981C9
-
-
2.6.1.97 Sulfurisphaera tokodaii
-
 gene arcS
-
6.3.4.20 Crenarchaeota
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
 Sulfurisphaera tokodaii L-glutamate + archaeine15 in tRNA i.e. G+, whcih is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
 Euryarchaeota L-glutamate + archaeine15 in tRNA i.e. G+, which is found only in Archaea at position 15 in the dihydrouridine loop, i.e. D-loop ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O i.e. preQ0-tRNA Sulfurisphaera tokodaii L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O i.e. preQ0-tRNA Euryarchaeota L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 additional information in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulting deletion strain is transformed with an expression plasmid containing GAT-queC from Ssulfolobus solfataricus (SSO0016) cloned behind a PBAD promoter. Peaks corresponding to 7-amidino-7-deazaguanosine (archaeosine) and 7-cyano-7-deazaguanine nucleoside are detected in tRNA extracted from the strain expressing SSO0016 Saccharolobus solfataricus ?
-
 ?
2.6.1.97 additional information in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulting deletion strain is transformed with an expression plasmid containing GAT-queC from Ssulfolobus solfataricus (SSO0016) cloned behind a PBAD promoter. Peaks corresponding to 7-amidino-7-deazaguanosine (archaeosine) and 7-cyano-7-deazaguanine nucleoside are detected in tRNA extracted from the strain expressing SSO0016 Saccharolobus solfataricus P2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.97 ARChaeosine synthase
-
 Sulfurisphaera tokodaii
2.6.1.97 ARChaeosine synthase
-
 Euryarchaeota
2.6.1.97 ArcS
-
 Sulfurisphaera tokodaii
2.6.1.97 ArcS
-
 Euryarchaeota
2.6.1.97 ATP-independent amidinotransferase
-
 Sulfurisphaera tokodaii
2.6.1.97 ATP-independent amidinotransferase
-
 Euryarchaeota
2.6.1.97 SSO0016 locus name Saccharolobus solfataricus
6.3.4.20 queC
-
 Crenarchaeota

General Information

EC Number General Information Comment Organism
2.6.1.97 evolution specific Archaea such as Sulfolobus tokodaii have retained ArcS in addition to GAT-QueC, overview Sulfurisphaera tokodaii
2.6.1.97 metabolism GAT-QueC also catalyzes biosynthesis of G+-tRNA, pathways, overview Sulfurisphaera tokodaii
2.6.1.97 additional information G+ can be tolerated in Escherichia coli at position 34 in normally Q-containing tRNA when recombinant GAT-QueC and QueF are introduced Euryarchaeota
2.6.1.97 physiological function ArcS catalyzes the final step in the G+ pathway, the conversion of preQ0-tRNA to G+-tRNA, in Euryarchaeota Sulfurisphaera tokodaii
2.6.1.97 physiological function ArcS catalyzes the final step in the G+ pathway, the conversion of preQ0-tRNA to G+-tRNA, in Euryarchaeota Euryarchaeota
6.3.4.20 evolution the enzyme belongs to GAT-QueC, a two-domain family with an N-terminal glutamine amidotransferase class-II domain fused to a domain homologous to QueC, the enzyme that produces preQ0. Phylogenetic distribution of aTGT, ArcS, GAT-QueC, and QueF-like in the two archaeal phyla, overview. Most crenarchaeal genomes encode a fused GAT-QueC protein or a QueF-like protein Crenarchaeota
6.3.4.20 physiological function the enzyme QueC produces the G+ precursor, 7-cyano-7-deazaguanine, i.e. preQ0, which is inserted into tRNA by tRNA-guanine transglycosylase before conversion into G+ by archaeosine synthase, ArcS. Gat-QueC and QueF-like families can compensate for lack of ArcS, overview Crenarchaeota