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Literature summary extracted from

  • Irimia, A.; Loukachevitch, L.V.; Eoff, R.L.; Guengerich, F.P.; Egli, M.
    Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus (2010), Acta Crystallogr. Sect. F, 66, 1013-1018.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 expressed in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.7 determination of crystal structures of a binary Mg2+-form Dpo4–DNA complex with 1,N2-etheno-dG in the template strand as well as of ternary Mg2+-form Dpo4–DNA–dCTP/dGTP complexes with 8-oxoG in the template strand. Crystals are grown using the sitting-drop vapor-diffusion method by mixing equal amounts of Dpo4–DNA complex solution and of a reservoir solution containing 12–20% polyethylene glycol 3350, 0.2 M ammonium acetate, 0.1 M magnesium acetate and 20 mMTris pH 7.5 Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Saccharolobus solfataricus Q97W02
-
-
2.7.7.7 Saccharolobus solfataricus P2 Q97W02
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase Dpo4
-
 Saccharolobus solfataricus