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Literature summary extracted from

  • Srisimarat, W.; Kaulpiboon, J.; Krusong, K.; Zimmermann, W.; Pongsawasdi, P.
    Altered large-ring cyclodextrin product profile due to a mutation at Tyr-172 in the amylomaltase of Corynebacterium glutamicum (2012), Appl. Environ. Microbiol., 78, 7223-7228.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expression in Escherichia coli Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.25 Y172A mutant exhibits lower disproportionation, cyclization, and hydrolysis activities than the wild-type. The kcat/Km of the disproportionation reaction for the Y172A enzyme is 2.8fold lower than that of wild-type. The Y172A enzyme shows a product pattern different from that of wild-type at a long incubation time. The principal large-ring cyclodextrin products of the Y172A mutant are a cycloamylose mixture with a degree of polymerization of 28 or 29 Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 12.9
-
 maltotriose mutant Y172A, pH 6.0, 30°C Corynebacterium glutamicum
2.4.1.25 19.6
-
 maltotriose wild-type, pH 6.0, 30°C Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 81000
-
 x * 81000, SDS-PAGE Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Corynebacterium glutamicum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.25 6.2
-
 mutant Y172A, pH 6.0, 30°C Corynebacterium glutamicum
2.4.1.25 21.8
-
 wild-type, pH 6.0, 30°C Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltotriose
-
 Corynebacterium glutamicum maltooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 ? x * 81000, SDS-PAGE Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 30
-
-
 Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 50
-
 10 min, almost complete loss of activity Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.25 0.036
-
 maltotriose mutant Y172A, pH 6.0, 30°C Corynebacterium glutamicum
2.4.1.25 0.157
-
 maltotriose wild-type, pH 6.0, 30°C Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6
-
-
 Corynebacterium glutamicum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.25 5.5 9 stable at 30°C Corynebacterium glutamicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.25 0.0028
-
 maltotriose mutant Y172A, pH 6.0, 30°C Corynebacterium glutamicum
2.4.1.25 0.0078
-
 maltotriose wild-type, pH 6.0, 30°C Corynebacterium glutamicum