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Literature summary extracted from

  • Guagliardi, A.; Cerchia, L.; Rossi, M.
    An intracellular protease of the crenarchaeon Sulfolobus solfataricus, which has sequence similarity to eukaryotic peptidases of the CD clan (2002), Biochem. J., 368, 357-363.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B77 DTNB 5 mM, 49% inhibition Saccharolobus solfataricus
3.4.22.B77 additional information no inhibition by 5 mM EDTA, 0.01 mM pepstatin A or 0.1 mM E-64 Saccharolobus solfataricus
3.4.22.B77 N-ethylmaleimide 5 mM, 95% inhibition Saccharolobus solfataricus
3.4.22.B77 PMSF 3 mM, 16% inhibition Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.B77 intracellular
-
 Saccharolobus solfataricus 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B77 10000
-
 gel filtration Saccharolobus solfataricus
3.4.22.B77 11072
-
 1 * 11072, calculated from sequence Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B77 Saccharolobus solfataricus
-
-
-
3.4.22.B77 Saccharolobus solfataricus P2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B77
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B77 Bacillus stearothermophilus alcohol dehydrogenase + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.4.22.B77 Bacillus stearothermophilus alcohol dehydrogenase + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
3.4.22.B77 L-Glu-7-amido-4-methylcoumarin + H2O
-
 Saccharolobus solfataricus L-Glu + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 L-Glu-7-amido-4-methylcoumarin + H2O
-
 Saccharolobus solfataricus P2 L-Glu + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin + H2O 14% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-acetyl-Asp-Glu-Val-Asp + 7-amino-4-methylcoumarin + H2O
-
 ?
3.4.22.B77 N-benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O 7.1% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O 9.5% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-benzyloxycarbonyl-Gly-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus P2 N-succinyl-Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-succinyl-Ala-Glu-7-amido-4-methylcoumarin + H2O 81% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-succinyl-Ala-Glu + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O 11% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-succinyl-Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O 11% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus P2 N-succinyl-Ala-Phe-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.22.B77 N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O 12% of the hydrolysis rate compared to L-Glu-7-amido-4-methylcoumarin Saccharolobus solfataricus P2 N-succinyl-Leu-Leu-Val-Tyr + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.B77 monomer 1 * 10000, SDS-PAGE Saccharolobus solfataricus
3.4.22.B77 monomer 1 * 11072, calculated from sequence Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B77 peptidase C61.001
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.B77 70
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.B77 60 80 60°C: about 75% of maximal activity, 80°C: about 50% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.B77 70
-
 90 min, prior heating of the protease solution stimulats the hydrolysis rate Saccharolobus solfataricus
3.4.22.B77 80
-
 30 min, prior heating of the protease solution stimulats the hydrolysis rate Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.B77 7.5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.B77 7 8.5 pH 7.0: about 80% of maximal activity, pH 8.5: about 40% of maximal activity Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.B77 Saccharolobus solfataricus calculated from sequence
-
 10.5