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Literature summary extracted from

  • Wolterink-van Loo, S.; van Eerde, A.; Siemerink, M.A.; Akerboom, J.; Dijkstra, B.W.; van der Oost, J.
    Biochemical and structural exploration of the catalytic capacity of Sulfolobus KDG aldolases (2007), Biochem. J., 403, 421-430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.55 overexpression in Escherichia Sulfolobus acidocaldarius
4.1.2.55 overexpression in Escherichia Sulfurisphaera tokodaii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.55 hanging-drop method, the enzyme crystallizes in two space groups, P3(1)21 and P6(5)22, for which diffraction data are obtained to a maximum resolution of 1.8 and 2.5 A Sulfolobus acidocaldarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.55 0.7
-
 pyruvate pH 6.0, 70°C Sulfurisphaera tokodaii
4.1.2.55 1.1
-
 pyruvate pH 6.0, 70°C Sulfolobus acidocaldarius
4.1.2.55 3.9
-
 D-glyceraldehyde pH 6.0, 70°C Sulfurisphaera tokodaii
4.1.2.55 6.3
-
 D-glyceraldehyde pH 6.0, 70°C Sulfolobus acidocaldarius

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.55 Sulfolobus acidocaldarius Q4JC35
-
-
4.1.2.55 Sulfurisphaera tokodaii F9VPG1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.55
-
 Sulfolobus acidocaldarius
4.1.2.55
-
 Sulfurisphaera tokodaii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.55 additional information the enzyme shows strict requirement for pyruvate Sulfolobus acidocaldarius ?
-
 ?
4.1.2.55 pyruvate + D-glyceraldehyde higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-dehydro-3-deoxygluconate and D-2-dehydro-3-deoxy-galactonate Sulfurisphaera tokodaii 2-dehydro-3-deoxy-D-galactonate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-galactonate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-dehydro-3-deoxygluconate and D-2-dehydro-3-deoxy-galactonate Sulfurisphaera tokodaii 2-dehydro-3-deoxy-D-gluconate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde. The enzyme forms a mixture of D-2-keto-3-deoxygluconate and D-2-keto-3-deoxy-galactonate Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-gluconate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde 3-phosphate higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-galactonate 6-phosphate
-
 r
4.1.2.55 pyruvate + D-glyceraldehyde 3-phosphate higher activity with D-glyceraldehyde 3-phosphate compared to D-glyceraldehyde Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-gluconate 6-phosphate
-
 r
4.1.2.55 pyruvate + glycolaldehyde
-
 Sulfolobus acidocaldarius 2-dehydro-3-deoxy-D-xylonate
-
 r
4.1.2.55 pyruvate + glycolaldehyde
-
 Sulfolobus acidocaldarius 2-dehydro-3-deoxy-L-arabinonate
-
 r
4.1.2.55 Pyruvate + glyoxylate
-
 Sulfolobus acidocaldarius ?
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.55 70
-
 assay at Sulfolobus acidocaldarius
4.1.2.55 70
-
 assay at Sulfurisphaera tokodaii
4.1.2.55 90
-
-
 Sulfurisphaera tokodaii
4.1.2.55 99
-
-
 Sulfolobus acidocaldarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.55 100
-
 t1/2: 110 min Sulfurisphaera tokodaii
4.1.2.55 100
-
 t1/2: 18.9 min Sulfolobus acidocaldarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.55 5.5
-
-
 Sulfurisphaera tokodaii
4.1.2.55 6
-
 assay at Sulfolobus acidocaldarius
4.1.2.55 6
-
 assay at Sulfurisphaera tokodaii
4.1.2.55 6.5
-
-
 Sulfolobus acidocaldarius