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Literature summary extracted from

  • Hanner, M.; Redl, B.; Stöffler, G.
    Isolation and characterization of an intracellular aminopeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus (1990), Biochim. Biophys. Acta, 1033, 148-153.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.B3 acetic acid 1%, 86% inhibition Saccharolobus solfataricus
3.4.11.B3 acetonitrile 10%, 26% inhibition Saccharolobus solfataricus
3.4.11.B3 bestatin 1 mM, 97% inhibition Saccharolobus solfataricus
3.4.11.B3 EDTA 1 mM, complete inhibition Saccharolobus solfataricus
3.4.11.B3 ethanol 10%, complete inhibition Saccharolobus solfataricus
3.4.11.B3 mercaptoethanol 1%, 56% inhibition Saccharolobus solfataricus
3.4.11.B3 additional information no inhibition by PMSF, TLCK or soybean trypsin inhibitor Saccharolobus solfataricus
3.4.11.B3 SDS 0.1%, complete inhibition Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.B3 0.1
-
L-leucyl 4-nitroanilide pH 7.0, 70°C Saccharolobus solfataricus
3.4.11.B3 0.21
-
L-alanyl 4-nitroanilide pH 7.0, 70°C Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.B3 intracellular
-
Saccharolobus solfataricus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.B3 Co2+ 1 mM, restores enzyme activity after inhibition with EDTA Saccharolobus solfataricus
3.4.11.B3 Mn2+ 1 mM, restores enzyme activity after inhibition with EDTA Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.B3 80000
-
4 * 80000, SDS-PAGE Saccharolobus solfataricus
3.4.11.B3 320000
-
gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.B3 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.B3
-
Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.B3 0.225
-
pH 7.0, 70°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.B3 glycyl 4-nitroanilide + H2O 11% of the activity compared to L-Leu-4-nitroanilide Saccharolobus solfataricus glycine + 4-nitroaniline
-
?
3.4.11.B3 L-alanyl 4-nitroanilide + H2O 118% of the activity compared to L-Leu-4-nitroanilide Saccharolobus solfataricus L-alanine + 4-nitroaniline
-
?
3.4.11.B3 L-arginyl 4-nitroanilide + H2O 63% of the activity compared to L-Leu-4-nitroanilide Saccharolobus solfataricus L-arginine + 4-nitroaniline
-
?
3.4.11.B3 L-leucyl 4-nitroanilide + H2O
-
Saccharolobus solfataricus L-leucine + 4-nitroaniline
-
?
3.4.11.B3 L-lysyl 4-nitroanilide + H2O 15% of the activity compared to L-Leu-4-nitroanilide Saccharolobus solfataricus L-lysine + 4-nitroaniline
-
?
3.4.11.B3 L-phenylalanyl 4-nitroanilide + H2O 62% of the activity compared to L-Leu-4-nitroanilide Saccharolobus solfataricus L-phenylalanine + 4-nitroaniline
-
?
3.4.11.B3 additional information no activity with N-acetyl-L-phenylalanine, N-acetyl-L-leucine, N-benzoyl-L-arginine Saccharolobus solfataricus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.B3 homotetramer 4 * 80000, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.B3 intracellular Sulfolobus aminopeptidase
-
Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.B3 75
-
adition of 0.05 mM each of Co2+, Mn2+, Mg2+, Ca2+ and Zn2+ has no effect on the temperature optimum. At higher concentrations of divalent metal ions a decrease of activity occurs Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.B3 60 90 60°C: about 60% of maximal activity, 90°C: about 35% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.B3 75
-
15 min, activity decreases to 10% in absence of Co2+, in presence of 0.05 mM the remaining activity is 76% Saccharolobus solfataricus
3.4.11.B3 85
-
15 min, complete loss of activity in absence of Co2+, in presence of 0.05 mM the remaining activity is 60% Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.B3 6.8
-
-
Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.B3 5.5 7.5 pH 5.5: about 45% of maximal activity, pH 7.5: about 65% of maximal activity Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.B3 3 9 14 h, at least 70% residual activity Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.B3 Saccharolobus solfataricus isoelectric focusing
-
4.4