Literature summary extracted from
Hu, Y.; Peng, N.; Han, W.; Mei, Y.; Chen, Z.; Feng, X.; Liang, Y.X.; She, Q.
An archaeal protein evolutionarily conserved in prokaryotes is a zinc-dependent metalloprotease (2012), Biosci. Rep., 32, 609-618.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.4.24.B29 |
leupeptin |
1 mM, 1.7fold activation |
Saccharolobus solfataricus |
|
3.4.24.B29 |
PMSF |
5 mM, 1.2fold activation |
Saccharolobus solfataricus |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.24.B29 |
overexpression in Escherichia coli |
Saccharolobus solfataricus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.24.B29 |
C416G |
4% of the activity compared to wild-type enzyme, azocasein as substrate |
Saccharolobus solfataricus |
3.4.24.B29 |
E229D |
11% of the activity compared to wild-type enzyme, azocasein as substrate |
Saccharolobus solfataricus |
3.4.24.B29 |
H228F |
8% of the activity compared to wild-type enzyme, azocasein as substrate |
Saccharolobus solfataricus |
3.4.24.B29 |
H233Y |
14% of the activity compared to wild-type enzyme, azocasein as substrate |
Saccharolobus solfataricus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.24.B29 |
1,10-phenanthroline |
10 mM, 90% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
Co2+ |
5 mM, 91% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
Cu2+ |
5 mM, 37% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
E-64 |
0.01 mM, 44% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
EDTA |
5 mM, complete inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
Fe2+ |
5 mM, 93% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
Hg2+ |
5 mM, 15% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
Mg2+ |
5 mM, 97% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
Mn2+ |
5 mM, 37% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
N-ethylmaleimide |
5 mM, 64% inhibition |
Saccharolobus solfataricus |
|
3.4.24.B29 |
Sr2+ |
5 mM, 83% inhibition |
Saccharolobus solfataricus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.24.B29 |
Zn2+ |
5 mM, stimulates activity up to 160%, contains a zinc-binding motif |
Saccharolobus solfataricus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.4.24.B29 |
55000 |
- |
2 * 55000, the protein forms disulfide bond via the Cys416 residue, yielding protein dimer that is the active form of the enzyme, SDS-PAGE |
Saccharolobus solfataricus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.24.B29 |
Saccharolobus solfataricus |
Q7LXP6 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.24.B29 |
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.24.B29 |
azocasein + H2O |
- |
Saccharolobus solfataricus |
fragments of azocasein |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.24.B29 |
dimer |
2 * 55000, the protein forms disulfide bond via the Cys416 residue, yielding protein dimer that is the active form of the enzyme, SDS-PAGE |
Saccharolobus solfataricus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.24.B29 |
Sso0660 |
- |
Saccharolobus solfataricus |
3.4.24.B29 |
TldD |
- |
Saccharolobus solfataricus |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.24.B29 |
55 |
60 |
- |
Saccharolobus solfataricus |
Temperature Range [°C]
EC Number |
Temperature Minimum [°C] |
Temperature Maximum [°C] |
Comment |
Organism |
---|
3.4.24.B29 |
45 |
85 |
45°C: about 85% of maximal activity, 85°C: about 60% of maximal activity |
Saccharolobus solfataricus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.24.B29 |
7 |
- |
- |
Saccharolobus solfataricus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.4.24.B29 |
6 |
9 |
6: more than 75% of maximal activity, pH 9: about 80% of maximal activity |
Saccharolobus solfataricus |