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Literature summary extracted from

  • Del Vecchio, P.; Elias, M.; Merone, L.; Graziano, G.; Dupuy, J.; Mandrich, L.; Carullo, P.; Fournier, B.; Rochu, D.; Rossi, M.; Masson, P.; Chabriere, E.; Manco, G.
    Structural determinants of the high thermal stability of SsoPox from the hyperthermophilic archaeon Sulfolobus solfataricus (2009), Extremophiles, 13, 461-470.
    View publication on PubMed

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.8.1 guanidine-HCl the conformational stability of SsoPox against the denaturing action of guanidine-HCl has been investigated at 25°C, pH 8.0, 20 mM Tris–HCl buffer, by performing CD and fluorescence measurements. The transition curves obtained by recording the molar ellipticity at 222 nm (detecting the secondary structure stability) present two inflection points, at 2.6 M guanidine-HCl and about 4.8 M guanidine-HCl, respectively, with a plateau at about 3 M guanidine-HCl. SsoPox is markedly more resistant to the denaturing action of guanidine-HCl with respect to the mesophilic counterpart. Overall guanidine-HCl-induced denaturation of SsoPox is not a reversible process in all the investigated experimental conditions: upon suitable dilution of fully denatured samples, there is not a complete recovery of the far-UV CD spectrum or fluorescence emission spectrum of the native enzyme Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.1 Saccharolobus solfataricus Q97VT7
-
-
3.1.8.1 Saccharolobus solfataricus P2 Q97VT7
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.8.1 the conformational stability of SsoPox against the denaturing action of guanidine-HCl has been investigated at 25°C, pH 8.0, 20 mM Tris–HCl buffer, by performing CD and fluorescence measurements. The transition curves obtained by recording the molar ellipticity at 222 nm (detecting the secondary structure stability) present two inflection points, at 2.6 M guanidine-HCl and about 4.8 M guanidine-HCl, respectively, with a plateau at about 3 M guanidine-HCl. SsoPox is markedly more resistant to the denaturing action of guanidine-HCl with respect to the mesophilic counterpart. Overall guanidine-HCl-induced denaturation of SsoPox is not a reversible process in all the investigated experimental conditions: upon suitable dilution of fully denatured samples, there is not a complete recovery of the far-UV CD spectrum or fluorescence emission spectrum of the native enzyme Saccharolobus solfataricus

Subunits

EC Number Subunits Comment Organism
3.1.8.1 dimer two fundamental differences between SsoPox and the mesophilic counterparts are: (a) a larger number of surface salt bridges, also involved in complex networks; (b) a tighter quaternary structure due to an optimization of the interactions at the interface between the two monomers Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 POX
-
 Saccharolobus solfataricus
3.1.8.1 Sso
-
 Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.8.1 additional information
-
 highly thermostable enzyme Saccharolobus solfataricus
3.1.8.1 106
-
 estimated denaturation temperature Saccharolobus solfataricus