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Literature summary extracted from

  • dAbusco, A.S.; Ammendola, S.; Scandurra, R.; Politi, L.
    Molecular and biochemical characterization of the recombinant amidase from hyperthermophilic archaeon Sulfolobus solfataricus (2001), Extremophiles, 5, 183-192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.4 overexpression in Escherichia coli as a fusion protein with an N-terminus six-histidine-residue affinity tag Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.3
-
 nicotinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.3
-
 Benzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.3
-
 cyclomaltohexaose pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.3
-
 2-toluamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.3
-
 4-Aminobenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.3
-
 2-Aminobenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.4
-
 2-hydroxybenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.8
-
 Isonicotinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.9
-
 3-indolacetamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1
-
 Isobutyramide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.2
-
 butyramide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.2
-
 methacrylamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.3
-
 Propionamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.3
-
 Pentanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.5
-
 4-hydroxybenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 2.5
-
 picolinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 3
-
 Acrylamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 3
-
 lactamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 3.6
-
 acetamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 4
-
 3-phenylpropionamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 5
-
 cyclohexanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 5.8
-
 hexanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 6.7
-
 L-prolinamide pH 7.5, 70°C Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.4 Co2+ 1 mM, about 20% activation Saccharolobus solfataricus
3.5.1.4 Fe3+ 1 mM, about 20% activation Saccharolobus solfataricus
3.5.1.4 additional information no effect: Cu2+, Mn2+, and Ca2+ Saccharolobus solfataricus
3.5.1.4 V2+ 1 mM, about 20% activation Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 55000
-
 x * 55000, SDS-PAGE Saccharolobus solfataricus
3.5.1.4 55784
-
 x * 55784, calculated from sequence Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Saccharolobus solfataricus P95896
-
-
3.5.1.4 Saccharolobus solfataricus MT4 P95896
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4
-
 Saccharolobus solfataricus

Storage Stability

EC Number Storage Stability Organism
3.5.1.4 the enzyme may be stored at room temperature in the presence of 0.04% NaN3 for 1 year without any loss of activity Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 (S)-2-phenylpropionamide + H2O no activity with (R)-2-phenylpropionamide Saccharolobus solfataricus (S)-2-phenylpropionate + NH3
-
 ?
3.5.1.4 2-aminobenzamide + H2O
-
 Saccharolobus solfataricus 2-aminobenzoic acid + NH3
-
 ?
3.5.1.4 2-hydroxybenzamide + H2O
-
 Saccharolobus solfataricus 2-hydroxybenzoic acid + NH3
-
 ?
3.5.1.4 2-thiophenecarboxamide + H2O
-
 Saccharolobus solfataricus 2-thiophenecarboxylate + NH3
-
 ?
3.5.1.4 2-toluamide + H2O
-
 Saccharolobus solfataricus 2-toluic acid + NH3
-
 ?
3.5.1.4 3-indolacetamide + H2O
-
 Saccharolobus solfataricus 3-indolacetate + NH3
-
 ?
3.5.1.4 3-phenylpropionamide + H2O
-
 Saccharolobus solfataricus 3-phenylpropionate + NH3
-
 ?
3.5.1.4 3-toluamide + H2O
-
 Saccharolobus solfataricus 3-toluic acid + NH3
-
 ?
3.5.1.4 4-aminobenzamide + H2O
-
 Saccharolobus solfataricus 4-aminobenzoic acid + NH3
-
 ?
3.5.1.4 4-hydroxybenzamide + H2O
-
 Saccharolobus solfataricus 4-hydroxybenzoic acid + NH3
-
 ?
3.5.1.4 4-methylbenzamide + H2O best aromatic substrate Saccharolobus solfataricus 4-methylbenzoic acid + NH3
-
 ?
3.5.1.4 4-toluamide + H2O
-
 Saccharolobus solfataricus 4-toluic acid + NH3
-
 ?
3.5.1.4 acetamide + H2O
-
 Saccharolobus solfataricus acetate + NH3
-
 ?
3.5.1.4 acrylamide + H2O
-
 Saccharolobus solfataricus acrylate + NH3
-
 ?
3.5.1.4 anthranilamide + H2O
-
 Saccharolobus solfataricus anthranilate + NH3
-
 ?
3.5.1.4 benzamide + H2O
-
 Saccharolobus solfataricus benzoate + NH3
-
 ?
3.5.1.4 butyramide + H2O
-
 Saccharolobus solfataricus butyrate + NH3
-
 ?
3.5.1.4 cyclohexanamide + H2O
-
 Saccharolobus solfataricus cyclohexanoate + NH3
-
 ?
3.5.1.4 hexanamide + H2O
-
 Saccharolobus solfataricus hexanoate + NH3
-
 ?
3.5.1.4 isobutyramide + H2O best aliphatic substrate Saccharolobus solfataricus isobutyrate + NH3
-
 ?
3.5.1.4 isobutyramide + H2O best aliphatic substrate Saccharolobus solfataricus MT4 isobutyrate + NH3
-
 ?
3.5.1.4 isonicotinamide + H2O
-
 Saccharolobus solfataricus isonicotinic acid + NH3
-
 ?
3.5.1.4 L-asparagine + H2O
-
 Saccharolobus solfataricus L-aspartate + NH3
-
 ?
3.5.1.4 L-asparagine + H2O
-
 Saccharolobus solfataricus MT4 L-aspartate + NH3
-
 ?
3.5.1.4 L-glutamine + H2O
-
 Saccharolobus solfataricus L-glutamate + NH3
-
 ?
3.5.1.4 L-glutamine + H2O
-
 Saccharolobus solfataricus MT4 L-glutamate + NH3
-
 ?
3.5.1.4 L-prolinamide + H2O
-
 Saccharolobus solfataricus L-proline + NH3
-
 ?
3.5.1.4 lactamide + H2O
-
 Saccharolobus solfataricus lactate + NH3
-
 ?
3.5.1.4 methacrylamide + H2O
-
 Saccharolobus solfataricus methacrylate + NH3
-
 ?
3.5.1.4 additional information the enzyme transfers the acyl group also to hydroxylamine and hydrazine Saccharolobus solfataricus ?
-
 ?
3.5.1.4 additional information the enzyme transfers the acyl group also to hydroxylamine and hydrazine Saccharolobus solfataricus MT4 ?
-
 ?
3.5.1.4 nicotinamide + H2O
-
 Saccharolobus solfataricus nicotinic acid + NH3
-
 ?
3.5.1.4 pentanamide + H2O
-
 Saccharolobus solfataricus pentanoate + NH3
-
 ?
3.5.1.4 pentanamide + H2O
-
 Saccharolobus solfataricus MT4 pentanoate + NH3
-
 ?
3.5.1.4 picolinamide + H2O
-
 Saccharolobus solfataricus picolinate + NH3
-
 ?
3.5.1.4 propionamide + H2O
-
 Saccharolobus solfataricus propionate + NH3
-
 ?
3.5.1.4 pyrazinamide + H2O
-
 Saccharolobus solfataricus pyrazinoic acid + NH3
-
 ?
3.5.1.4 salicylamide + H2O
-
 Saccharolobus solfataricus salicylate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 ? x * 55000, SDS-PAGE Saccharolobus solfataricus
3.5.1.4 ? x * 55784, calculated from sequence Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 70
-
 assay at Saccharolobus solfataricus
3.5.1.4 95
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.4 75 95 75°C: about 50% of maximal activity, 95°C: maximal activity, 100°C: less than 10% of activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.4 70
-
 activity is constant for more than 72 h Saccharolobus solfataricus
3.5.1.4 80
-
 half-life: 25 h Saccharolobus solfataricus
3.5.1.4 90
-
 half-life: 0.5 h Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 0.3
-
 2-toluamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.8
-
 4-Aminobenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.9
-
 2-Aminobenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.1
-
 4-hydroxybenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.3
-
 2-hydroxybenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.6
-
 nicotinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.6
-
 acetamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.6
-
 Isonicotinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.8
-
 picolinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.8
-
 3-indolacetamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 2.9
-
 methacrylamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 3.1
-
 L-prolinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 3.2
-
 Propionamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 5.3
-
 hexanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 6.4
-
 Benzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 7.1
-
 3-phenylpropionamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 7.3
-
 4-toluamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 8.3
-
 Acrylamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 9.9
-
 butyramide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 10.7
-
 lactamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 16.3
-
 cyclohexanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 20.5
-
 Pentanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 21.9
-
 Isobutyramide pH 7.5, 70°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 7.5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 4 9.5 pH 4: about 50% of maximal activity, pH 9.5: about 50% of maximal activity Saccharolobus solfataricus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.4 Saccharolobus solfataricus protein without His-tag tail calculated from amino acid composition
-
 5.94
3.5.1.4 Saccharolobus solfataricus protein with His-tag tail calculated from amino acid composition
-
 6.05

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.4 0.27
-
 4-Aminobenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.3
-
 2-Aminobenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.44
-
 acetamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.46
-
 L-prolinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.72
-
 picolinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.73
-
 4-hydroxybenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.75
-
 2-toluamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 0.91
-
 hexanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 1.8
-
 3-phenylpropionamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 2
-
 Isonicotinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 2
-
 3-indolacetamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 2.4
-
 methacrylamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 2.46
-
 Propionamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 2.8
-
 Acrylamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 3.3
-
 2-hydroxybenzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 3.3
-
 cyclohexanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 3.57
-
 lactamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 5.3
-
 nicotinamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 8.25
-
 butyramide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 15.7
-
 Pentanamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 21.3
-
 Benzamide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 21.9
-
 Isobutyramide pH 7.5, 70°C Saccharolobus solfataricus
3.5.1.4 24.3
-
 cyclomaltohexaose pH 7.5, 70°C Saccharolobus solfataricus