EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 0.009 | - |
isocitrate | pH 8.0, 55°C, cofactor: NADP+ | Saccharolobus solfataricus | |
1.1.1.42 | 0.085 | - |
NADP+ | pH 8.0, 55°C | Saccharolobus solfataricus | |
1.1.1.42 | 0.11 | - |
NADP+ | pH 8.0, 30°C | Haloferax volcanii | |
1.1.1.42 | 0.13 | - |
isocitrate | pH 8.0, 30°C, cofactor: NADP+ | Haloferax volcanii | |
1.1.1.42 | 2.9 | - |
NAD+ | pH 8.0, 55°C | Saccharolobus solfataricus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | KCl | the activity of the enzyme is markedly dependent on the concentration of NaCl or KC1 in the Tris/EDTA/Mg2+ buffer, being maximal in 0.5 M NaCl or KCl. The stimulatory effect of KCl is greater than that of NaCl | Haloferax volcanii | |
1.1.1.42 | NaCl | the activity of the enzyme is markedly dependent on the concentration of NaCl or KC1 in the Tris/EDTA/Mg2+ buffer, being maximal in 0.5 M NaCl or KCl. The stimulatory effect of KCl is greater than that of NaCl | Haloferax volcanii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 45000 | - |
2 * 45000, SDS-PAGE | Saccharolobus solfataricus |
1.1.1.42 | 62000 | - |
2 * 62000, SDS-PAGE | Haloferax volcanii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | Haloferax volcanii | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Saccharolobus solfataricus | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Saccharolobus solfataricus DSM 1616 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Haloferax volcanii DSM 3757 | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.42 | Haloferax volcanii | - |
- |
- |
1.1.1.42 | Haloferax volcanii DSM 3757 | - |
- |
- |
1.1.1.42 | Saccharolobus solfataricus | Q9UWG7 | - |
- |
1.1.1.42 | Saccharolobus solfataricus DSM 1616 | Q9UWG7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.42 | - |
Haloferax volcanii |
1.1.1.42 | - |
Saccharolobus solfataricus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 40 | - |
pH 8.0, 30°C | Haloferax volcanii |
1.1.1.42 | 152 | - |
pH 8.0, 55°C | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NAD+ | Km-value for NAD+ is 34fold higher compared to KM-value for NADP+ | Saccharolobus solfataricus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.42 | isocitrate + NAD+ | Km-value for NAD+ is 34fold higher compared to KM-value for NADP+ | Saccharolobus solfataricus DSM 1616 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Haloferax volcanii | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Saccharolobus solfataricus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Km-value for NAD+ is 34fold higher compared to KM-value for NADP+ | Saccharolobus solfataricus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Saccharolobus solfataricus DSM 1616 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | Km-value for NAD+ is 34fold higher compared to KM-value for NADP+ | Saccharolobus solfataricus DSM 1616 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
1.1.1.42 | isocitrate + NADP+ | - |
Haloferax volcanii DSM 3757 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.42 | dimer | 2 * 45000, SDS-PAGE | Saccharolobus solfataricus |
1.1.1.42 | dimer | 2 * 62000, SDS-PAGE | Haloferax volcanii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 30 | - |
assay at | Haloferax volcanii |
1.1.1.42 | 55 | - |
assay at | Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | additional information | - |
the enzyme is incubated in Tris/EDTA/Mg2+ buffer containing either 0.5 M or 3 M KCl. The thermal stability of the enzyme is substantially reduced at the lower KCl concentration, with concomitant differences in the activation energies for the thermal inactivation process. Omission of Mg2+ from the incubation buffer greatly accelerates the rate of loss of activity, and its presence plus that of 3 M KCl are recommended for storage of the enzyme | Haloferax volcanii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 8 | - |
assay at | Haloferax volcanii |
1.1.1.42 | 8 | - |
assay at | Saccharolobus solfataricus |