Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lower, B.H.; Bischoff, K.M.; Kennelly, P.J.
    The archaeon Sulfolobus solfataricus contains a membrane-associated protein kinase activity that preferentially phosphorylates threonine residues in vitro (2000), J. Bacteriol., 182, 3452-3459.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.1 1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine i.e. ML-9, 0.5-1.0 mM, 50% inhibition Saccharolobus solfataricus
2.7.11.1 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine i.e. H-7 Saccharolobus solfataricus
2.7.11.1 genistein
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.1 0.00074
-
 ATP pH 6.5, 25°C Saccharolobus solfataricus
2.7.11.1 0.013
-
 KKRAARATSNVFA pH 6.5, 25°C, presence of poly(Glu4:Tyr) Saccharolobus solfataricus
2.7.11.1 0.0157
-
 ATP pH 6.5, 65°C Saccharolobus solfataricus
2.7.11.1 0.02
-
 KKRAARASSNVFA pH 6.5, 65°C Saccharolobus solfataricus
2.7.11.1 0.03
-
 KKRAARATSNVFA pH 6.5, 25°C Saccharolobus solfataricus
2.7.11.1 0.045
-
 KKRAARATSNVFA pH 6.5, 65°C Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.1 membrane
-
 Saccharolobus solfataricus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.1 Mg2+ potential divalent metal ion cofactors Mn2+ and Mg2+ Saccharolobus solfataricus
2.7.11.1 Mn2+ potential divalent metal ion cofactors Mn2+ and Mg2+ Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.1 67000
-
 2 * 67000, SDS-PAGE Saccharolobus solfataricus
2.7.11.1 125000
-
 gel filtration Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.1 Saccharolobus solfataricus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.1 phosphoprotein the protein kinase undergoes autophosphorylation on threonine Saccharolobus solfataricus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.1 partial Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.1 0.0024
-
 pH 6.5, 25°C, substrate: casein Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.1 ADP + casein phosphorylated on threonine residues Saccharolobus solfataricus AMP + phosphorylated casein
-
 ?
2.7.11.1 ATP + casein phosphorylated on threonine residues Saccharolobus solfataricus ADP + phosphorylated casein
-
 ?
2.7.11.1 ATP + histone H4 phosphorylated on threonine and on serine residues Saccharolobus solfataricus ADP + phosphorylated histone H4
-
 ?
2.7.11.1 ATP + KKRAARASSNVFA phosphorylation of the peptide is undetectable at 25°C. Phosphorylation takes place at measurable rates if the assay temperature is raised to 65°C or if poly(Glu4:Tyr) is included in the assay mixture Saccharolobus solfataricus ADP + ?
-
 ?
2.7.11.1 ATP + KKRAARATSNVFA
-
 Saccharolobus solfataricus ADP + KKRAARApTSNVFA
-
 ?
2.7.11.1 ATP + reduced carboxyamidomethylated and maleylated lysozyme phosphorylated on threonine residues Saccharolobus solfataricus ADP + phosphorylated reduced carboxyamidomethylated and maleylated lysozyme
-
 ?
2.7.11.1 GDP + casein phosphorylated on threonine residues Saccharolobus solfataricus GMP + phosphorylated casein
-
 ?
2.7.11.1 GTP + casein phosphorylated on threonine residues Saccharolobus solfataricus GDP + phosphorylated casein
-
 ?
2.7.11.1 additional information neither of the pyrimidine nucleotide triphosphates tested, CTP and UTP, serves as substrates and neither does AMP, GMP, or pyrophosphate Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.1 dimer 2 * 67000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.1 25
-
 assay at Saccharolobus solfataricus
2.7.11.1 65
-
 assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.1 6.5
-
 assay at Saccharolobus solfataricus