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Literature summary extracted from

  • Moonen, M.J.; Synowsky, S.A.; van den Berg, W.A.; Westphal, A.H.; Heck, A.J.; van den Heuvel, R.H.; Fraaije, M.W.; van Berkel, W.J.
    Hydroquinone dioxygenase from Pseudomonas fluorescens ACB: a novel member of the family of nonheme-iron(II)-dependent dioxygenases (2008), J. Bacteriol., 190, 5199-5209.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.66 additional information ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.66 2,2'-dipyridyl inactivation Pseudomonas fluorescens
1.13.11.66 4-hydroxybenzoate competitive with hydroquinone Pseudomonas fluorescens
1.13.11.66 4-Hydroxybenzonitrile 0.2 mM, no residual activity Pseudomonas fluorescens
1.13.11.66 4-hydroxycinnamate 0.2 mM, no residual activity Pseudomonas fluorescens
1.13.11.66 4-nitrophenol 0.2 mM, no residual activity Pseudomonas fluorescens
1.13.11.66 bromohydroquinone substrate inhibition Pseudomonas fluorescens
1.13.11.66 chlorohydroquinone substrate inhibition Pseudomonas fluorescens
1.13.11.66 hydrogen peroxide inactivation Pseudomonas fluorescens
1.13.11.66 Hydroxyhydroquinone 0.2 mM, 9% residual activity Pseudomonas fluorescens
1.13.11.66 methoxyhydroquinone strong substrtae inhibition Pseudomonas fluorescens
1.13.11.66 additional information weak or no inhibition: 2-hydroxy-, 3-hydroxy-, 2,3-dihydroxy-, 2,5-dihydroxy-, 2,6-dihydroxy-, 3,4-dihydroxy-, 3,4,5-trihydroxy-, 3-chloro-4-hydroxy-, tetrafluoro-4-hydroxy-, 3-amino-4-hydroxy-, 4-hydroxy-3-methoxy-, 4-amino- and methyl 4-hydroxybenzoate; 6-hydroxynicotinate, 4-hydroxypropiophenone, 4-hydroxymandelate, 4-hydroxyphenylglycine, 4-hydroxybenzenesulfonic acid, and 4-methyl-, 4-methoxy-, and 4-aminophenol Pseudomonas fluorescens
1.13.11.66 o-phenanthroline inactivation Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.66 0.037
-
 hydroquinone pH 7.0, 25°C Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.66 Iron 10fold activation by Fe2+, each subunit binds one molecule of 4-hydroxybenzoate and one molecule of iron Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.66 17800
-
 2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit Pseudomonas fluorescens
1.13.11.66 38300
-
 2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit Pseudomonas fluorescens
1.13.11.66 112000
-
 gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.66 Pseudomonas fluorescens
-
-
-
1.13.11.66 Pseudomonas fluorescens ACB
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.66 ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.66 5.89
-
 pH 7.0, 25°C Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.66 2,3-difluorohydroquinone + O2 80% of the activity with hydroquinone Pseudomonas fluorescens ?
-
 ?
1.13.11.66 2,3-difluorohydroquinone + O2 80% of the activity with hydroquinone Pseudomonas fluorescens ACB ?
-
 ?
1.13.11.66 2,5-difluorohydroquinone + O2 75% of the activity with hydroquinone Pseudomonas fluorescens ?
-
 ?
1.13.11.66 2,5-difluorohydroquinone + O2 75% of the activity with hydroquinone Pseudomonas fluorescens ACB ?
-
 ?
1.13.11.66 3,5-difluorohydroquinone + O2 90% of the activity with hydroquinone Pseudomonas fluorescens ?
-
 ?
1.13.11.66 3,5-difluorohydroquinone + O2 90% of the activity with hydroquinone Pseudomonas fluorescens ACB ?
-
 ?
1.13.11.66 bromohydroquinone + O2 30% of the activity with hydroquinone Pseudomonas fluorescens ?
-
 ?
1.13.11.66 bromohydroquinone + O2 30% of the activity with hydroquinone Pseudomonas fluorescens ACB ?
-
 ?
1.13.11.66 chlorohydroquinone + O2 70% of the activity with hydroquinone Pseudomonas fluorescens ?
-
 ?
1.13.11.66 hydroquinone + O2
-
 Pseudomonas fluorescens 4-hydroxymuconic semialdehyde
-
 ?
1.13.11.66 methoxyhydroquinone + O2 50% of the activity with hydroquinone Pseudomonas fluorescens ?
-
 ?
1.13.11.66 methylhydroquinone + O2 120% of the activity with hydroquinone Pseudomonas fluorescens ?
-
 ?
1.13.11.66 additional information no substrate: tetrafluorohydroquinone, 1,2,4-trihydroxybenzene, gentisate, catechol, resorcinol, pyrogallol, and phenol Pseudomonas fluorescens ?
-
 ?
1.13.11.66 additional information no substrate: tetrafluorohydroquinone, 1,2,4-trihydroxybenzene, gentisate, catechol, resorcinol, pyrogallol, and phenol Pseudomonas fluorescens ACB ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.66 tetramer 2 * 17800, alpha-subunit, + 2 * 38300, beta-subunit Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.66 2.1
-
 hydroquinone pH 7.0, 25°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.66 7 8
-
 Pseudomonas fluorescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.66 0.014
-
 4-hydroxybenzoate pH 7.0, 25°C Pseudomonas fluorescens