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Literature summary extracted from

  • Tan, Y.; Neo, P.C.; Najimudin, N.; Sudesh, K.; Muhammad, T.S.; Othman, A.S.; Samian, R.
    Cloning and characterization of poly(3-hydroxybutyrate) biosynthesis genes from Pseudomonas sp. USM 4-55 (2010), J. Basic Microbiol., 50, 179-189.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.304 gene phbCPs encoded in the phb operon, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, the genetic organization of phb operon shows a putative promoter region, followed by phbBPs-phbAPs-phbCPs, with phbRPs encoding a putative transcriptional activator that is located in the opposite orientation, upstream of phbBACPs. Heterologous expression of phbCPs from pGEM3ABex vector in Escherichia coli JM109 resulting in P(3HB) accumulation of up to 40% of dry cell weight Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.304 62300
-
 x * 62300, about, sequence calculation Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Pseudomonas sp. C0KTZ0 isolated in Malaysia, gene phbC encoded in the phb operon
-
2.3.1.304 Pseudomonas sp. USM4-55 C0KTZ0 isolated in Malaysia, gene phbC encoded in the phb operon
-

Subunits

EC Number Subunits Comment Organism
2.3.1.304 ? x * 62300, about, sequence calculation Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 P(3HB) synthase
-
 Pseudomonas sp.
2.3.1.304 PhbC
-
 Pseudomonas sp.

General Information

EC Number General Information Comment Organism
2.3.1.304 physiological function P(3HB) synthase catalyzes polymerization of the 3-hydroxybutyryl-CoA monomers, Pseudomonas sp. USM 4-55 is a soil isolated bacterium that possesses the ability to produce polyhydroxyalkanoates consisting of both poly(3-hydroxybutyrate) homopolymer and medium-chain length monomers (6 to 14 carbon atoms) when sugars or fatty acids are utilized as the sole carbon source Pseudomonas sp.