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Literature summary extracted from

  • Hossain, M.; Nakano, R.; Nakamura, K.; Kimura, Y.
    Molecular identification and characterization of an acidic peptide:N-glycanase from tomato (Lycopersicum esculentum) fruits (2010), J. Biochem., 147, 157-165.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.52 expressed in Escherichia coli strain JM109 and in Pichia pastoris strain GS115 Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.52 additional information the enzyme does not require any metal ions for full enzymatic activity Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.52 65000
-
 x * 65000, SDS-PAGE Solanum lycopersicum
3.5.1.52 65800
-
 x * 65800, calculated from amino acid sequence Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.52 additional information Solanum lycopersicum the enzyme hydrolyses the beta-aspartyl-glycosylamine bond of N-linked glycoproteins/glycopeptides and releases free N-glycans ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.52 Solanum lycopersicum D0QU16 cultivar Momotaro
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.52 glycoprotein the enzyme possesses 10 N-glycosylation sites at positions of 220, 266, 386, 397, 447, 481, 489, 551, 556 and 570 in the deduced polypeptide chain Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.52 Ni-nitrilotriacetate column chromatography Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.52 fruit
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.52 Cys-Gly-Leu-Val-Pro-Val-Leu-Ala-Glu-Asn-Tyr-Asn(Man3Gal2GlcNAc4NeuAc2)-Lys + H2O
-
 Solanum lycopersicum ?
-
 ?
3.5.1.52 human transferrin glycopeptide + H2O
-
 Solanum lycopersicum ?
-
 ?
3.5.1.52 additional information the enzyme hydrolyses the beta-aspartyl-glycosylamine bond of N-linked glycoproteins/glycopeptides and releases free N-glycans Solanum lycopersicum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.52 ? x * 65000, SDS-PAGE Solanum lycopersicum
3.5.1.52 ? x * 65800, calculated from amino acid sequence Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.5.1.52 acidic peptide:N-glycanase
-
 Solanum lycopersicum
3.5.1.52 acidic PNGase
-
 Solanum lycopersicum
3.5.1.52 glycoamidase
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.52 40
-
-
 Solanum lycopersicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.52 20 60 enzyme activity declines rapidly above 40°C (80% activity at 50°C, 55% activity at 60°C, 30% activity at 70°C, no activity at 80°C) Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.52 4.5
-
-
 Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.52 3 6 enzyme activity declines rapidly above pH 6.0 Solanum lycopersicum

General Information

EC Number General Information Comment Organism
3.5.1.52 physiological function the enzyme is involved in a different de-N-glycosylation mechanism associated with plant growth and development Solanum lycopersicum