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Literature summary extracted from
- The aglycone specificity-determining sites are different in 2, 4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA)-glucosidase (Maize beta -glucosidase) and dhurrinase (Sorghum beta -glucosidase) (2000), J. Biol. Chem., 275, 20002-20011.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | expressed in Escherichia coli as a His-tagged fusion protein | Zea mays |
| 3.2.1.21 | expressed in Escherichia coli as a His-tagged fusion protein | Sorghum bicolor |
| 3.2.1.182 | expressed in Escherichia coli as a His-tagged fusion protein | Zea mays |
| 3.2.1.182 | expressed in Escherichia coli as a His-tagged fusion protein | Sorghum bicolor |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | additional information | to study the mechanism of substrate specificity further, eight chimeric beta-glucosidases are constructed by replacing peptide sequences within the C-terminal region of Zea mays Glu1 with the homologous peptide sequences of Sorghum Dhr1 or vice versa. Replacing the peptide 466FAGFTERY473 of Zea mays Glu1 with the homologous peptide 462SSGYTERF469 of Sorghum Dhr1 or replacing the peptide 481NNNCTRYMKE490 in Glu1 with the homologous peptide 477ENGCERTMKR486 of Dhr1 is sufficient to confer to Zea mays Glu1 the ability to hydrolyze dhurrin | Zea mays |
| 3.2.1.21 | additional information | to study the mechanism of substrate specificity further, eight chimeric beta-glucosidases are constructed by replacing peptide sequences within the C-terminal region of Zea mays Glu1 with the homologous peptide sequences of Sorghum Dhr1 or vice versa. Replacing the peptide 466FAGFTERY473 of Zea mays Glu1 with the homologous peptide 462SSGYTERF469 of Sorghum Dhr1 or replacing the peptide 481NNNCTRYMKE490 in Glu1 with the homologous peptide 477ENGCERTMKR486 of Dhr1 is sufficient to confer to Zea mays Glu1 the ability to hydrolyze dhurrin | Sorghum bicolor |
| 3.2.1.182 | additional information | to study the mechanism of substrate specificity further, eight chimeric beta-glucosidases are constructed by replacing peptide sequences within the C-terminal region of Zea mays Glu1 with the homologous peptide sequences of Sorghum Dhr1 or vice versa. Replacing the peptide 466FAGFTERY473 of Zea mays Glu1 with the homologous peptide 462SSGYTERF469 of Sorghum Dhr1 or replacing the peptide 481NNNCTRYMKE490 in Glu1 with the homologous peptide 477ENGCERTMKR486 of Dhr1 is sufficient to confer to Zea mays Glu1 the ability to hydrolyze dhurrin | Zea mays |
| 3.2.1.182 | additional information | to study the mechanism of substrate specificity further, eight chimeric beta-glucosidases are constructed by replacing peptide sequences within the C-terminal region of Zea mays Glu1 with the homologous peptide sequences of Sorghum Dhr1 or vice versa. Replacing the peptide 466FAGFTERY473 of Zea mays Glu1 with the homologous peptide 462SSGYTERF469 of Sorghum Dhr1 or replacing the peptide 481NNNCTRYMKE490 in Glu1 with the homologous peptide 477ENGCERTMKR486 of Dhr1 is sufficient to confer to Zea mays Glu1 the ability to hydrolyze dhurrin | Sorghum bicolor |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.04 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim23 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 492-508 of Zea mays) | Zea mays |  |
| 3.2.1.21 | 0.04 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim23 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 492-508 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 0.05 | - |
Dhurrin | pH 5.8, 37°C, wild-type | Sorghum bicolor | |
| 3.2.1.21 | 0.09 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.09 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.1 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.1 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.12 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim39 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 466-492 of Zea mays) | Zea mays | |
| 3.2.1.21 | 0.12 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim39 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 466-492 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 0.15 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim17 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 462-508 of Zea mays) | Zea mays | |
| 3.2.1.21 | 0.15 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim17 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 462-508 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 0.18 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.18 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.35 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.35 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.35 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.35 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.36 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.36 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.36 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.36 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.38 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, wild-type | Zea mays | |
| 3.2.1.21 | 0.39 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.39 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.42 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.42 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 0.51 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 0.51 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 1.02 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 1.02 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 1.23 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 1.23 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 1.3 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, wild-type | Zea mays | |
| 3.2.1.21 | 1.36 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 1.36 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 1.39 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 1.39 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 1.4 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 1.4 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 0.098 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 0.098 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 0.098 | - |
DIMBOA-glucoside | pH 5.8, 37°C, wild-type | Zea mays | |
| 3.2.1.182 | 0.104 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 0.104 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 0.106 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 0.106 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 0.2 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 0.2 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 0.205 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 0.205 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Sorghum bicolor | - |
- |
- |
| 3.2.1.21 | Zea mays | - |
- |
- |
| 3.2.1.182 | Sorghum bicolor | - |
- |
- |
| 3.2.1.182 | Zea mays | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | using Ni-NTA chromatography | Zea mays |
| 3.2.1.21 | using Ni-NTA chromatography | Sorghum bicolor |
| 3.2.1.182 | using Ni-NTA chromatography | Zea mays |
| 3.2.1.182 | using Ni-NTA chromatography | Sorghum bicolor |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Zea mays | 2-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Sorghum bicolor | 2-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Zea mays | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Sorghum bicolor | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | dhurrin + H2O | - |
Zea mays | (2S)-hydroxy(4-hydroxyphenyl)ethanenitrile + beta-D-glucose | - |
? | |
| 3.2.1.21 | dhurrin + H2O | - |
Sorghum bicolor | (2S)-hydroxy(4-hydroxyphenyl)ethanenitrile + beta-D-glucose | - |
? | |
| 3.2.1.182 | DIMBOA-glucoside + H2O | - |
Zea mays | DIMBOA + beta-D-glucose | - |
? | |
| 3.2.1.182 | DIMBOA-glucoside + H2O | - |
Sorghum bicolor | DIMBOA + beta-D-glucose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | SbDhr1 | - |
Sorghum bicolor |
| 3.2.1.21 | ZmGlu1 | - |
Zea mays |
| 3.2.1.182 | SbDhr1 | - |
Sorghum bicolor |
| 3.2.1.182 | ZmGlu1 | - |
Zea mays |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 25 | - |
assay at 25°C, substrates: 2-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-glucopyranoside | Zea mays |
| 3.2.1.21 | 25 | - |
assay at 25°C, substrates: 2-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-glucopyranoside | Sorghum bicolor |
| 3.2.1.21 | 37 | - |
assay at 37°C, substrates: dhurrin and DIMBOA-glucose | Zea mays |
| 3.2.1.21 | 37 | - |
assay at 37°C, substrates: dhurrin and DIMBOA-glucose | Sorghum bicolor |
| 3.2.1.182 | 25 | - |
assay at 25°C, substrates: 2-nitrophenyl-beta-D-glucopyranoside and 4-nitrophenyl-beta-D-glucopyranoside | Sorghum bicolor |
| 3.2.1.182 | 37 | - |
assay at 37°C, substrates: dhurrin and DIMBOA-glucoside | Zea mays |
| 3.2.1.182 | 37 | - |
assay at 37°C, substrates: dhurrin and DIMBOA-glucoside | Sorghum bicolor |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 13.46 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 13.46 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 18.68 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim17 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 462-508 of Zea mays) | Zea mays | |
| 3.2.1.21 | 18.68 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim17 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 462-508 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 19.83 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, wild-type | Zea mays | |
| 3.2.1.21 | 22.7 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 22.7 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 23.41 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim39 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 466-492 of Zea mays) | Zea mays | |
| 3.2.1.21 | 23.41 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim39 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 466-492 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 24.16 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, wild-type | Zea mays | |
| 3.2.1.21 | 27.17 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 27.17 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 31.19 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 31.19 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 31.83 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 31.83 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 34.26 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 34.26 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 37.05 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 37.05 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 45.65 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 45.65 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 46.02 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 46.02 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 53.96 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim23 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 492-508 of Zea mays) | Zea mays | |
| 3.2.1.21 | 53.96 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim23 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 492-508 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 54.02 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 54.02 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 64.25 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 64.25 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 68.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 68.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 77.7 | - |
Dhurrin | pH 5.8, 37°C, wild-type | Sorghum bicolor | |
| 3.2.1.21 | 87.24 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 87.24 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 90.8 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 90.8 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 107.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 107.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 19.54 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 19.54 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 20.15 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 20.15 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 24.02 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 24.02 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 25.67 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 25.67 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 29.16 | - |
DIMBOA-glucoside | pH 5.8, 37°C, wild-type | Zea mays | |
| 3.2.1.182 | 43.33 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 43.33 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5.8 | - |
assay at | Zea mays |
| 3.2.1.21 | 5.8 | - |
assay at | Sorghum bicolor |
| 3.2.1.182 | 5.8 | - |
assay at | Zea mays |
| 3.2.1.182 | 5.8 | - |
assay at | Sorghum bicolor |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 15.25 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, wild-type | Zea mays | |
| 3.2.1.21 | 22.43 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 22.43 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 26.1 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 26.1 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 26.46 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 26.46 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 43.91 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 43.91 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 44.75 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 44.75 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 47.24 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 47.24 | - |
2-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 62.4 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 62.4 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 63.57 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, wild-type | Zea mays | |
| 3.2.1.21 | 97.88 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 97.88 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 124 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim17 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 462-508 of Zea mays) | Zea mays | |
| 3.2.1.21 | 124 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim17 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 462-508 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 150 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 150 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 195 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim39 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 466-492 of Zea mays) | Zea mays | |
| 3.2.1.21 | 195 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim39 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 466-492 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 196.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 196.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 216.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 216.4 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 242.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 242.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 276.7 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 276.7 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.8, 25°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 303.1 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 303.1 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 494.8 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.21 | 494.8 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.21 | 1349 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim23 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 492-508 of Zea mays) | Zea mays | |
| 3.2.1.21 | 1349 | - |
Dhurrin | pH 5.8, 37°C, mutant Chim23 (chimeric construct: parental Sorghum Dhr1 with exchanged domain 492-508 of Zea mays) | Sorghum bicolor | |
| 3.2.1.21 | 1494 | - |
Dhurrin | pH 5.8, 37°C, wild-type | Sorghum bicolor | |
| 3.2.1.182 | 97.7 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 97.7 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim16 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-495 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 195.2 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 195.2 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim21 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-473 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 211 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 211 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim2 (chimeric construct: parental Zea mays Glu1 with exchanged domain 466-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 226 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 226 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim15 (chimeric construct: parental Zea mays Glu1 with exchanged domain 496-518 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 261.9 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Zea mays | |
| 3.2.1.182 | 261.9 | - |
DIMBOA-glucoside | pH 5.8, 37°C, mutant Chim22 (chimeric construct: parental Zea mays Glu1 with exchanged domain 481-490 of Sorghum Dhr1) | Sorghum bicolor | |
| 3.2.1.182 | 297.5 | - |
DIMBOA-glucoside | pH 5.8, 37°C, wild-type | Zea mays | |
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Release 2023.1 (January 2023)
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