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Literature summary extracted from

  • Roberts, J.A.; White, M.F.
    An archaeal endonuclease displays key properties of both eukaryal XPF-ERCC1 and Mus81 (2005), J. Biol. Chem., 280, 5924-5928.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.99.B2 Saccharolobus solfataricus Q7LXL5
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-
3.1.99.B2 Saccharolobus solfataricus P2 Q7LXL5
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.99.B2 additional information the best substrate is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The efficient cleavage of a nicked duplex substrate demonstrates that no single-stranded flap is required, rather a discontinuity in the DNA duplex is enough to promote the nuclease activity Saccharolobus solfataricus ?
-
?
3.1.99.B2 additional information the best substrate is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The efficient cleavage of a nicked duplex substrate demonstrates that no single-stranded flap is required, rather a discontinuity in the DNA duplex is enough to promote the nuclease activity Saccharolobus solfataricus P2 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.99.B2 XPF endonuclease
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Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.99.B2 55
-
assay at Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.99.B2 7.6
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assay at Saccharolobus solfataricus