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Literature summary extracted from

  • Eoff, R.L.; Irimia, A.; Egli, M.; Guengerich, F.P.
    Sulfolobus solfataricus DNA polymerase Dpo4 is partially inhibited by "wobble" pairing between O6-methylguanine and cytosine, but accurate bypass is preferred (2007), J. Biol. Chem., 282, 1456-1467.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.7 crystallization of enzyme-DNA complexes Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.7 additional information
-
 additional information steady-state kinetic parameters for one-base incorporation Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Saccharolobus solfataricus Q97W02
-
-
2.7.7.7 Saccharolobus solfataricus P2 Q97W02
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn the rate-constant defining Dpo4-catalyzed incorporation of dCTP is about 6-fold slower for incorporation opposite O6-MeG relative to G. The basis for the decreased rate is revealed by the crystal structure to be formation of a wobble base pairing between O6-MeG and C Saccharolobus solfataricus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn the rate-constant defining Dpo4-catalyzed incorporation of dCTP is about 6-fold slower for incorporation opposite O6-MeG relative to G. The basis for the decreased rate is revealed by the crystal structure to be formation of a wobble base pairing between O6-MeG and C Saccharolobus solfataricus P2 diphosphate + DNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase Dpo4
-
 Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.7 additional information
-
 additional information steady-state kinetic parameters for one-base incorporation Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.7 additional information
-
 additional information steady-state kinetic parameters for one-base incorporation Saccharolobus solfataricus