EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.47 | W66F | mutation changes midpoint potential from -361 mV for wild-type to -427 mV. Mutant displays 2.5fold lower activity when reaction is supported by flavoproteins or NADPH instead of tetrahydrofolate | Bacillus subtilis |
1.14.14.47 | W66H | mutation changes midpoint potential from -361 mV for wild-type to -302 mV. Activity is similar to wild-type | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.47 | Iron | in the presence of L-arginine and tetrahydrofolate, wild-type and mutants W66F and W66H exist in the typical Fe(III) high-spin configuration | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.47 | Bacillus subtilis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.47 | 2 L-arginine + 3 reduced flavodoxin + 4 O2 | - |
Bacillus subtilis | 2 L-citrulline + 2 nitric oxide + 3 oxidized flavodoxin + 4 H2O | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.47 | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin | - |
Bacillus subtilis | |
1.14.14.47 | additional information | enzyme is able to use several different redox partners | Bacillus subtilis | |
1.14.14.47 | NADPH | - |
Bacillus subtilis | |
1.14.14.47 | tetrahydrofolate | - |
Bacillus subtilis |