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Literature summary extracted from

  • Zerbe, P.; Chiang, A.; Yuen, M.; Hamberger, B.; Hamberger, B.; Draper, J.A.; Britton, R.; Bohlmann, J.
    Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production (2012), J. Biol. Chem., 287, 12121-12131.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.140 recombinantly expressed in Escherichia coli Abies balsamea

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.140 homology modeling based on PDB entry 3p5pA. The active site residues Asp-48, Leu617, Phe696, and Gly723 are potentially important for the specificity Abies balsamea

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.140 D402A/D404A mutation results in complete loss of activity Abies balsamea
4.2.3.140 D402A/D404A monofunctional class II protein variant, complete loss of enzymatic activity with geranylgeranyl diphosphate as substrate Abies balsamea
4.2.3.140 D621A monofunctional class I protein variant, mutant produces trace amounts of copalyl diphosphate plus labda-13-en-8,15-diol Abies balsamea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.B25 chloroplast
-
 Abies balsamea 9507
-
4.2.3.140 chloroplast
-
 Abies balsamea 9507
-
5.5.1.12 chloroplast
-
 Abies balsamea 9507
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.B25 Abies balsamea H8ZM71
-
-
4.2.3.140 Abies balsamea H8ZM73
-
-
5.5.1.12 Abies balsamea H8ZM71
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.140 using Ni-NTA chromatography Abies balsamea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.B25 copalyl diphosphate
-
 Abies balsamea isopimara-8(14),15-diene + diphosphate single product ?
4.2.3.B25 additional information bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate to isopimara-7,15-diene Abies balsamea ?
-
 ?
4.2.3.140 (13E)-8alpha-hydroxylabd-13-en-15-yl diphosphate formation of cis-abienol proceeds via cyclization and hydroxylation at carbon C-8 of a postulated carbocation intermediate in the class II active site, followed by cleavage of the diphosphate group and termination of the reaction sequence without further cyclization in the class I active site Abies balsamea cis-abienol + diphosphate
-
 ?
4.2.3.140 additional information using (E,E,E)-geranylgeranyl diphosphate as a substrate AbdiTPS4 shows a unique single-peak product profile, which corresponds to cis-abienol 4 Abies balsamea ?
-
 ?
4.2.3.140 additional information bifunctional class I/II enzyme Abies balsamea ?
-
 ?
5.5.1.12 geranylgeranyl diphosphate
-
 Abies balsamea (+)-copalyl diphosphate
-
 ?
5.5.1.12 additional information bifunctional enzyme that catalyzes two sequential cyclizations of geranylgeranyl diphosphate to isopimara-7,15-diene Abies balsamea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.B25 TPS2
-
 Abies balsamea
4.2.3.140 AbCAS
-
 Abies balsamea
4.2.3.140 AbdiTPS4
-
 Abies balsamea
4.2.3.140 CAS
-
 Abies balsamea
4.2.3.140 cis-abienol synthase
-
 Abies balsamea
5.5.1.12 TPS2
-
 Abies balsamea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.140 30
-
 assay at Abies balsamea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.140 7.2
-
 assay at Abies balsamea