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Literature summary extracted from

  • Völlmecke, C.; Drees, S.L.; Reimann, J.; Albers, S.V.; Lübben, M.
    The ATPases CopA and CopB both contribute to copper resistance of the thermoacidophilic archaeon Sulfolobus solfataricus (2012), Microbiology, 158, 1622-1633.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.8 gene copA, phylogenetic analysis and sequence comparisons, recombinant expression of Sulfolobus solfataricus CopA in Escherichia coli strain DC194, deficient in the PIB-type copper export ATPase copA, yielding poor or partial complementation Saccharolobus solfataricus
7.2.2.8 gene copB, phylogenetic analysis and sequence comparisons, recombinant expression of Sulfolobus solfataricus CopB in Escherichia coli strain DC194, deficient in the PIB-type copper export ATPase copA, yielding poor or partial complementation Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.8 additional information disruption of gene copA via allelic replacement does not affect the sensitivity of Sulfolobus solfataricus to reactive oxygen species Saccharolobus solfataricus
7.2.2.8 additional information disruption of gene copB via allelic replacement does not affect the sensitivity of Sulfolobus solfataricus to reactive oxygen species Saccharolobus solfataricus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.2.2.8 membrane an integral membrane protein of cytoplasmic membranes Saccharolobus solfataricus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.8 Mg2+ required Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.2.2.8 ATP + H2O + Cu+[side 1] Saccharolobus solfataricus
-
ADP + phosphate + Cu+[side 2]
-
?
7.2.2.8 ATP + H2O + Cu+[side 1] Saccharolobus solfataricus PBL2025
-
ADP + phosphate + Cu+[side 2]
-
?

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.8 Saccharolobus solfataricus
-
gene copB
-
7.2.2.8 Saccharolobus solfataricus B2CQV8 gene copA
-
7.2.2.8 Saccharolobus solfataricus PBL2025
-
gene copB
-
7.2.2.8 Saccharolobus solfataricus PBL2025 B2CQV8 gene copA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.8 ATP + H2O + Cu+[side 1]
-
Saccharolobus solfataricus ADP + phosphate + Cu+[side 2]
-
?
7.2.2.8 ATP + H2O + Cu+[side 1]
-
Saccharolobus solfataricus PBL2025 ADP + phosphate + Cu+[side 2]
-
?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.8 CopA
-
Saccharolobus solfataricus
7.2.2.8 CopB
-
Saccharolobus solfataricus

Expression

EC Number Organism Comment Expression
7.2.2.8 Saccharolobus solfataricus an increase in the external copper concentration induces the enzyme up
7.2.2.9 Saccharolobus solfataricus synthesized at low levels under standard growth conditions (i.e. under copper limitation), and the expression levels increases after addition of copper up

General Information

EC Number General Information Comment Organism
7.2.2.8 evolution the enzyme belongs to the PIB-ATPase family, phylogenetic analysis and tree Saccharolobus solfataricus
7.2.2.8 physiological function CopA is an effective copper pump at low and high copper concentrations. The enzyme is not a strict prerequisite to the biosynthesis of the copper protein cytochrome oxidase. CopA and CopB act as resistance factors to copper ions at overlapping concentrations, both ATPases are involved in resistance to silver Saccharolobus solfataricus
7.2.2.8 physiological function CopB is a low-affinity copper export ATPase, which is only relevant if the media copper concentration is exceedingly high. The enzyme is not a strict prerequisite to the biosynthesis of the copper protein cytochrome oxidase. CopA and CopB act as resistance factors to copper ions at overlapping concentrations, both ATPases are involved in resistance to silver Saccharolobus solfataricus
7.2.2.9 malfunction deletion mutant analysis demonstrates that CopA is an effective copper pump at low and high copper concentrations. The DELTAcopA mutant shows highly reduced growth at 2 mM. High toxicity of copper to the DELTAcopA Saccharolobus solfataricus
7.2.2.9 malfunction deletion mutant analysis demonstrates that CopB is low-affinity copper export ATPase. For the DELTAcopB mutant strain 1.4 mM CuSO4 is needed to slow the onset and beginning of stationary phase at lower levels Saccharolobus solfataricus
7.2.2.9 physiological function CopA is an effective copper pump at low and high copper concentrations. CopA and CopB act as resistance factors to copper ions at overlapping concentrations. CopA is also involved in resistance to silver Saccharolobus solfataricus
7.2.2.9 physiological function CopB is low-affinity copper export ATPase. CopA and CopB act as resistance factors to copper ions at overlapping concentrations. CopB is also involved in resistance to silver Saccharolobus solfataricus