EC Number | Cloned (Comment) | Organism |
---|---|---|
7.3.2.1 | expression of HvPHT1;1 in Allium cepa epidermal cell plasma membranes from gateway-enabled pMDC83 vector with HvPHT1;1 being placed in-frame, upstream of the mGFP6 gene | Hordeum vulgare |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
7.3.2.1 | additional information | HvPHT1;1 activity analysis in Xenopus laevis oocytes via cRNA-injection, low external sodium concentration is critical for phosphate transporter characterization in Xenopus laevis oocytes | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
7.3.2.1 | additional information | - |
additional information | negative voltage Michaelis-Menten kinetics data, overview | Hordeum vulgare | |
7.3.2.1 | 0.0019 | - |
phosphate/out | pH 7.5, temperature not specified in the publication | Hordeum vulgare |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
7.3.2.1 | additional information | PHT1;1 subcellular localization study, overview | Hordeum vulgare | - |
- |
7.3.2.1 | plasma membrane | - |
Hordeum vulgare | 5886 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.1 | ATP + H2O + phosphate/out | Hordeum vulgare | proton-coupled phosphate transport | ADP + phosphate + phosphate/in | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.3.2.1 | Hordeum vulgare | Q8H6E0 | cv. Clipper and cv. Sahara | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
7.3.2.1 | additional information | cell-specific expression pattern of PHT1;1 | Hordeum vulgare | - |
7.3.2.1 | root | PHT1;1 is highly expressed in root segments with dense hairs and less root tips | Hordeum vulgare | - |
7.3.2.1 | root hair | - |
Hordeum vulgare | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.3.2.1 | ATP + H2O + phosphate/out | - |
Hordeum vulgare | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | ATP + H2O + phosphate/out | proton-coupled phosphate transport | Hordeum vulgare | ADP + phosphate + phosphate/in | - |
? | |
7.3.2.1 | additional information | HvPHT1;1 shows preferential selectivity for phosphate and arsenate, but no transport of the other oxyanions SO42- and NO3- | Hordeum vulgare | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.3.2.1 | HvPHT1;1 | - |
Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
7.3.2.1 | 6 | 7.5 | assay at | Hordeum vulgare |
EC Number | Organism | Comment | Expression |
---|---|---|---|
7.3.2.1 | Hordeum vulgare | the HvPHT1;1 gene is largely induced by phosphate starvation | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
7.3.2.1 | physiological function | HvPHT1;1 is a high-affinity phosphate transporter involved in uptake of phosphate from soil solution under low phosphate conditions | Hordeum vulgare |