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Literature summary extracted from

  • Jung, J.; Jung, T.; Seo, D.; Yoon, S.; Choi, H.; Park, B.; Park, C.; Woo, E.
    Structural and functional analysis of substrate recognition by the 250s loop in amylomaltase from Thermus brockianus (2011), Proteins, 79, 633-644.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25
-
 Thermus brockianus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.25 to 2.3 A resolution. Structure shows a pattern of conformational flexibility in the 250s loop with higher B-factor. The conformational flexibility of the loop may be involved in substrate binding in the GH77 family Thermus brockianus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.25 F251G mutation results in significantly lower glucose production but increased maltose production from maltopentose substrates, showing an altered substrate-binding affinity Thermus brockianus
2.4.1.25 Q256G mutation results in increased Km for maltotriose and a sharp decrease of the transglycosylation factor for maltose Thermus brockianus
2.4.1.25 W258G mutant shows neither cyclization nor coupling activity, suggesting that residue Trp258 plays an essential role in all catalytic activities including hydrolysis and transglycosylation activities Thermus brockianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.25 2
-
 maltotriose wild-type, pH 6.0, 70°C Thermus brockianus
2.4.1.25 5.8
-
 maltotriose mutant F251G, pH 6.0, 70°C Thermus brockianus
2.4.1.25 9.5
-
 maltotriose mutant W258G, pH 6.0, 70°C Thermus brockianus
2.4.1.25 165
-
 maltotriose mutant Q256G, pH 6.0, 70°C Thermus brockianus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Thermus brockianus Q2VJA0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltotriose
-
 Thermus brockianus maltooligosaccharides
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.25 0.0017
-
 maltotriose mutant F251G, pH 6.0, 70°C Thermus brockianus
2.4.1.25 0.005
-
 maltotriose mutant W258G, pH 6.0, 70°C Thermus brockianus
2.4.1.25 0.015
-
 maltotriose wild-type, pH 6.0, 70°C Thermus brockianus
2.4.1.25 0.08
-
 maltotriose mutant Q256G, pH 6.0, 70°C Thermus brockianus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.25 0.00035
-
 maltotriose mutant F251G, pH 6.0, 70°C Thermus brockianus
2.4.1.25 0.00048
-
 maltotriose mutant Q256G, pH 6.0, 70°C Thermus brockianus
2.4.1.25 0.00048
-
 maltotriose mutant W258G, pH 6.0, 70°C Thermus brockianus
2.4.1.25 0.007
-
 maltotriose wild-type, pH 6.0, 70°C Thermus brockianus