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Literature summary extracted from

  • Kanagawa, M.; Baba, S.; Ebihara, A.; Shinkai, A.; Hirotsu, K.; Mega, R.; Kim, K.; Kuramitsu, S.; Sampei, G.; Kawai, G.
    Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) from Thermus thermophilus HB8 (2010), Acta Crystallogr. Sect. F, 66, 893-898.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.8 structure in the unliganded form, in complex with IMP and in complex with GMP, to 2.1, 1.9 and 2.2 A resolution, respectively. The overall fold of the IMP complex is similar to that of the unliganded form, but the main-chain and side-chain atoms of the active site move to accommodate IMP. The overall folds of the IMP and GMP complexes are almost identical to each other. Enzyme belongs to group I Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.0039
-
hypoxanthine pH 7.9, 25°C Thermus thermophilus
2.4.2.8 0.0074
-
guanine pH 7.9, 25°C Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Thermus thermophilus Q5SLS3
-
-
2.4.2.8 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SLS3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus GMP + diphosphate
-
?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 GMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus IMP + diphosphate
-
?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 IMP + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.8 HGPRTase
-
Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 9.1
-
hypoxanthine pH 7.9, 25°C Thermus thermophilus
2.4.2.8 20
-
guanine pH 7.9, 25°C Thermus thermophilus