Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shibahara, T.; Satomura, T.; Kawakami, R.; Ohshima, T.; Sakuraba, H.
    Crystallization and preliminary X-ray analysis of a dye-linked D-lactate dehydrogenase from the aerobic hyperthermophilic archaeon Aeropyrum pernix (2011), Acta Crystallogr. Sect. F, 67, 1425-1427.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.28 gene APE_0487, expression in Escherichia coli strain Rosetta-gami 2 (DE3) Aeropyrum pernix
1.1.99.6 expression in Escherichia coli Aeropyrum pernix

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.28 purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml of 15 mg/ml protein in 10 mM potassium phosphate, pH 7.0, with 0.002 ml of reservoir solution containing 14.4% PEG 8000, 80 mM cacodylate pH 6.5, 160 mM calcium acetate and 20% glycerol, and equilibration against 0.1 ml of reservoir solution, 25°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution Aeropyrum pernix
1.1.99.6 hanging-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 63.4, b = 119.4, c = 70.2 A, beta = 112.0°, and diffract to 2.0 A resolution Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 59000
-
 1 * 59000, recombinant enzyme, SDS-PAGE Aeropyrum pernix
1.1.1.28 61000
-
 recombinant enzyme, gel filtration Aeropyrum pernix

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Aeropyrum pernix
-
 pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+ Aeropyrum pernix DSM 11879
-
 pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Aeropyrum pernix Q9YEU4 gene APE_0487; gene APE_0487
-
1.1.99.6 Aeropyrum pernix Q9YEU4
-
-
1.1.99.6 Aeropyrum pernix DSM 11879 Q9YEU4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28 recombinant enzyme from Escherichia coli strain Rosetta-gami 2 (DE3) by nickel affnity chromatography and gel filtration Aeropyrum pernix
1.1.99.6
-
 Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Aeropyrum pernix pyruvate + NADH + H+
-
 r
1.1.1.28 (R)-lactate + NAD+
-
 Aeropyrum pernix DSM 11879 pyruvate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.28 monomer 1 * 59000, recombinant enzyme, SDS-PAGE Aeropyrum pernix

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 DLDH
-
 Aeropyrum pernix
1.1.99.6 APE_0487
-
 Aeropyrum pernix
1.1.99.6 dye-linked D-lactate dehydrogenase
-
 Aeropyrum pernix

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NAD+
-
 Aeropyrum pernix
1.1.1.28 NADH
-
 Aeropyrum pernix