EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.47 | expression in Escherichia coli | Streptomyces lavendulae |
2.6.99.3 | expression in Escherichia coli | Streptomyces lavendulae |
6.3.3.5 | C-terminally His6-tagged enzyme is overexpression in Escherichia coli | Streptomyces lavendulae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.47 | 0.12 | - |
hydrogen sulfide | 30°C, pH 8.0 | Streptomyces lavendulae | |
2.5.1.47 | 22 | - |
thiosulfate | 30°C, pH 8.0 | Streptomyces lavendulae | |
2.6.99.3 | 9 | - |
Hydroxyurea | 30°C, pH 8.0 | Streptomyces lavendulae | |
6.3.3.5 | 0.023 | - |
ATP | pH 8.0, 30°C, cosubstrate: D-homocysteine | Streptomyces lavendulae | |
6.3.3.5 | 0.037 | - |
ATP | pH 8.0, 30°C, cosubstrate: beta-aminooxy-D-alanine | Streptomyces lavendulae | |
6.3.3.5 | 0.045 | - |
ATP | pH 8.0, 30°C, cosubstrate: D-2,4-diaminobutyrate | Streptomyces lavendulae | |
6.3.3.5 | 0.045 | - |
ATP | pH 8.0, 30°C, cosubstrate: D-ornithine | Streptomyces lavendulae | |
6.3.3.5 | 0.054 | - |
ATP | pH 8.0, 30°C, cosubstrate: D-homoserine | Streptomyces lavendulae | |
6.3.3.5 | 0.073 | - |
ATP | pH 8.0, 30°C, cosubstrate: O-ureido-D-serine | Streptomyces lavendulae | |
6.3.3.5 | 10 | - |
O-ureido-D-serine | pH 8.0, 30°C | Streptomyces lavendulae | |
6.3.3.5 | 19 | - |
D-homocysteine | pH 8.0, 30°C | Streptomyces lavendulae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.3.5 | Mg2+ | required | Streptomyces lavendulae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 36000 | - |
4 * 36000, SDS-PAGE | Streptomyces lavendulae |
2.5.1.47 | 126000 | - |
gel filtration | Streptomyces lavendulae |
2.6.99.3 | 36000 | - |
4 * 36000, SDS-PAGE | Streptomyces lavendulae |
2.6.99.3 | 126000 | - |
gel filtration | Streptomyces lavendulae |
6.3.3.5 | 26000 | - |
gel filtration | Streptomyces lavendulae |
6.3.3.5 | 34000 | - |
1 * 34000, calculated from sequence | Streptomyces lavendulae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.99.3 | O-acetyl-L-serine + hydroxyurea | Streptomyces lavendulae | the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species | O-ureido-L-serine + acetate | - |
? | |
2.6.99.3 | O-acetyl-L-serine + hydroxyurea | Streptomyces lavendulae ATCC 11924 | the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species | O-ureido-L-serine + acetate | - |
? | |
6.3.3.5 | O-ureido-D-serine + ATP + H2O | Streptomyces lavendulae | the enzyme is involved in the biosynthesis of the antituberculosis antibiotic D-cycloserine | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
6.3.3.5 | O-ureido-D-serine + ATP + H2O | Streptomyces lavendulae ATCC 11924 | the enzyme is involved in the biosynthesis of the antituberculosis antibiotic D-cycloserine | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.47 | Streptomyces lavendulae | D2Z027 | - |
- |
2.5.1.47 | Streptomyces lavendulae ATCC 11924 | D2Z027 | - |
- |
2.6.99.3 | Streptomyces lavendulae | D2Z027 | - |
- |
2.6.99.3 | Streptomyces lavendulae ATCC 11924 | D2Z027 | - |
- |
6.3.3.5 | Streptomyces lavendulae | D2Z030 | - |
- |
6.3.3.5 | Streptomyces lavendulae ATCC 11924 | D2Z030 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.47 | - |
Streptomyces lavendulae |
2.6.99.3 | - |
Streptomyces lavendulae |
6.3.3.5 | - |
Streptomyces lavendulae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.47 | O-acetyl-L-serine + hydrogen sulfide | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme catalyzes the reaction with O-acetyl-L-serine and hydroxyurea with 80fold lower catalytic efficiency | Streptomyces lavendulae | L-cysteine + acetate | - |
? | |
2.5.1.47 | O-acetyl-L-serine + hydrogen sulfide | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme catalyzes the reaction with O-acetyl-L-serine and hydroxyurea with 80fold lower catalytic efficiency | Streptomyces lavendulae ATCC 11924 | L-cysteine + acetate | - |
? | |
2.5.1.47 | O-acetyl-L-serine + L-homocysteine | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate | Streptomyces lavendulae | cystathionine + acetate | - |
? | |
2.5.1.47 | O-acetyl-L-serine + L-homocysteine | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate | Streptomyces lavendulae ATCC 11924 | cystathionine + acetate | - |
? | |
2.5.1.47 | O-acetyl-L-serine + thiosulfate | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate | Streptomyces lavendulae | S-sulfo-L-cysteine + acetate | - |
? | |
2.5.1.47 | O-acetyl-L-serine + thiosulfate | the enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate | Streptomyces lavendulae ATCC 11924 | S-sulfo-L-cysteine + acetate | - |
? | |
2.6.99.3 | O-acetyl-L-serine + hydroxyurea | the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species | Streptomyces lavendulae | O-ureido-L-serine + acetate | - |
? | |
2.6.99.3 | O-acetyl-L-serine + hydroxyurea | O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate. No activity with O3-acetyl-D-serine and L-serine. The enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme also catalyzes the reaction with O-acetyl-L-serine and hydrogen sulfide (with 80fold higher catalytic efficiency) | Streptomyces lavendulae | O-ureido-L-serine + acetate | - |
? | |
2.6.99.3 | O-acetyl-L-serine + hydroxyurea | the enzyme participates in the biosynthetic pathway of D-cycloserine, an antibiotic substance produced by several Streptomyces species | Streptomyces lavendulae ATCC 11924 | O-ureido-L-serine + acetate | - |
? | |
2.6.99.3 | O-acetyl-L-serine + hydroxyurea | O-ureido-L-serine i.e. (2S)-2-amino-3-[(carbamoylamino)oxy]propanoate. No activity with O3-acetyl-D-serine and L-serine. The enzyme prefers sulfide as the second substrate, followed by hydroxyurea, L-homocysteine, and thiosulfate. The enzyme also catalyzes the reaction with O-acetyl-L-serine and hydrogen sulfide (with 80fold higher catalytic efficiency) | Streptomyces lavendulae ATCC 11924 | O-ureido-L-serine + acetate | - |
? | |
6.3.3.5 | beta-aminooxy-D-alanine + ATP + H2O | - |
Streptomyces lavendulae | D-cycloserine + ? | - |
? | |
6.3.3.5 | beta-aminooxy-D-alanine + ATP + H2O | - |
Streptomyces lavendulae ATCC 11924 | D-cycloserine + ? | - |
? | |
6.3.3.5 | D-2,4-diaminobutyrate + ATP + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
6.3.3.5 | D-2,4-diaminobutyrate + ATP + H2O | - |
Streptomyces lavendulae ATCC 11924 | ? | - |
? | |
6.3.3.5 | D-homocysteine + ATP + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
6.3.3.5 | D-homocysteine + ATP + H2O | - |
Streptomyces lavendulae ATCC 11924 | ? | - |
? | |
6.3.3.5 | D-homoserine + ATP + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
6.3.3.5 | D-ornithine + ATP + H2O | - |
Streptomyces lavendulae | ? | - |
? | |
6.3.3.5 | O-ureido-D-serine + ATP + H2O | the enzyme is involved in the biosynthesis of the antituberculosis antibiotic D-cycloserine | Streptomyces lavendulae | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
6.3.3.5 | O-ureido-D-serine + ATP + H2O | O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate. No activity with O-ureido-L-serine | Streptomyces lavendulae | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
6.3.3.5 | O-ureido-D-serine + ATP + H2O | the enzyme is involved in the biosynthesis of the antituberculosis antibiotic D-cycloserine | Streptomyces lavendulae ATCC 11924 | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? | |
6.3.3.5 | O-ureido-D-serine + ATP + H2O | O-ureido-D-serine i.e. (2R)-2-amino-3-[(carbamoylamino)oxy]propanoate. No activity with O-ureido-L-serine | Streptomyces lavendulae ATCC 11924 | D-cycloserine + CO2 + ammonia + ADP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.47 | tetramer | 4 * 36000, SDS-PAGE | Streptomyces lavendulae |
2.6.99.3 | tetramer | 4 * 36000, SDS-PAGE | Streptomyces lavendulae |
6.3.3.5 | monomer | 1 * 34000, calculated from sequence | Streptomyces lavendulae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.47 | DcsD | - |
Streptomyces lavendulae |
2.6.99.3 | DcsD | - |
Streptomyces lavendulae |
6.3.3.5 | DcsG | - |
Streptomyces lavendulae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 30 | - |
assay at | Streptomyces lavendulae |
2.6.99.3 | 30 | - |
assay at | Streptomyces lavendulae |
6.3.3.5 | 30 | - |
assay at | Streptomyces lavendulae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.47 | 0.72 | - |
thiosulfate | 30°C, pH 8.0 | Streptomyces lavendulae | |
2.5.1.47 | 12 | - |
hydrogen sulfide | 30°C, pH 8.0 | Streptomyces lavendulae | |
2.6.99.3 | 11 | - |
Hydroxyurea | 30°C, pH 8.0 | Streptomyces lavendulae | |
6.3.3.5 | 860 | - |
D-homocysteine | pH 8.0, 30°C | Streptomyces lavendulae | |
6.3.3.5 | 2200 | - |
O-ureido-D-serine | pH 8.0, 30°C | Streptomyces lavendulae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.47 | 8 | - |
assay at | Streptomyces lavendulae |
2.6.99.3 | 8 | - |
assay at | Streptomyces lavendulae |
2.6.99.3 | 9 | - |
activity increases from pH 5.0 to pH 9.0 | Streptomyces lavendulae |
6.3.3.5 | 8 | - |
- |
Streptomyces lavendulae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.6.99.3 | 7.5 | 9 | activity at pH 7.5 is about 45% compared to activity at pH 9.0 | Streptomyces lavendulae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.47 | 0.033 | - |
thiosulfate | 30°C, pH 8.0 | Streptomyces lavendulae | |
2.5.1.47 | 0.14 | - |
O-acetyl-L-serine | 30°C, pH 8.0, cosubstrate: hydrogen sulfide | Streptomyces lavendulae | |
2.5.1.47 | 0.19 | - |
L-homocysteine | 30°C, pH 8.0 | Streptomyces lavendulae | |
2.5.1.47 | 100 | - |
hydrogen sulfide | 30°C, pH 8.0 | Streptomyces lavendulae | |
2.6.99.3 | 0.15 | - |
O-acetyl-L-serine | 30°C, pH 8.0, cosubstrate: hydroxyurea | Streptomyces lavendulae | |
2.6.99.3 | 1.2 | - |
Hydroxyurea | 30°C, pH 8.0 | Streptomyces lavendulae | |
6.3.3.5 | 0.67 | - |
D-ornithine | pH 8.0, 30°C | Streptomyces lavendulae | |
6.3.3.5 | 2.1 | - |
D-2,4-diaminobutyrate | pH 8.0, 30°C | Streptomyces lavendulae | |
6.3.3.5 | 6.1 | - |
D-homoserine | pH 8.0, 30°C | Streptomyces lavendulae | |
6.3.3.5 | 14 | - |
beta-aminooxy-D-alanine | pH 8.0, 30°C | Streptomyces lavendulae | |
6.3.3.5 | 45 | - |
D-homocysteine | pH 8.0, 30°C | Streptomyces lavendulae | |
6.3.3.5 | 210 | - |
O-ureido-D-serine | pH 8.0, 30°C | Streptomyces lavendulae |