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Literature summary extracted from

  • Zhang, L.; Xu, Q.; Zhan, S.; Li, Y.; Lin, H.; Sun, S.; Sha, L.; Hu, K.; Guan, X.; Shen, Y.
    A new NAD(H)-dependent meso-2,3-butanediol dehydrogenase from an industrially potential strain Serratia marcescens H30 (2014), Appl. Microbiol. Biotechnol., 98, 1175-1184.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B20 gene budC, sequence comparisons, expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B20 CaCl2
-
 Serratia marcescens
1.1.1.B20 Fe3+ inhibits diacetyl and (3S/3R)-acetoin reduction Serratia marcescens
1.1.1.B20 FeCl3
-
 Serratia marcescens
1.1.1.B20 NaCl
-
 Serratia marcescens
1.1.1.B20 NH4Cl
-
 Serratia marcescens
1.1.1.B20 ZnSO4
-
 Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B20 0.97
-
 (3R)-acetoin pH 8.0, 40°C Serratia marcescens
1.1.1.B20 3.3
-
 diacetyl pH 8.0, 40°C Serratia marcescens
1.1.1.B20 4.1
-
 meso-2,3-butanediol pH 5.0, 40°C Serratia marcescens
1.1.1.B20 31.2
-
 (2S,3S)-2,3-butanediol pH 5.0, 40°C Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.B20 Fe2+ enhances for oxidation and reduction reactions Serratia marcescens
1.1.1.B20 Mg2+ enhances diacetyl and (3S/3R)-acetoin reduction Serratia marcescens
1.1.1.B20 Mn2+ enhances diacetyl and (3S/3R)-acetoin reduction Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B20 Serratia marcescens H9XP47 gene budC
-
1.1.1.B20 Serratia marcescens H30 H9XP47 gene budC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B20 (2S,3S)-2,3-butanediol + NAD+ low activity Serratia marcescens (3S)-acetoin + NADH + H+
-
 r
1.1.1.B20 (2S,3S)-2,3-butanediol + NAD+ low activity Serratia marcescens H30 (3S)-acetoin + NADH + H+
-
 r
1.1.1.B20 (3R)-acetoin + NADH + H+
-
 Serratia marcescens meso-2,3-butanediol + NAD+
-
 r
1.1.1.B20 (3R)-acetoin + NADH + H+
-
 Serratia marcescens H30 meso-2,3-butanediol + NAD+
-
 r
1.1.1.B20 (3S)-acetoin + NADH + H+ at pH 9.0 Serratia marcescens (2S,3S)-2,3-butanediol + NAD+
-
 r
1.1.1.B20 (3S)-acetoin + NADH + H+ at pH 9.0 Serratia marcescens H30 (2S,3S)-2,3-butanediol + NAD+
-
 r
1.1.1.B20 1,2-pentanediol + NADH + H+ low activity Serratia marcescens ?
-
 ?
1.1.1.B20 1,2-pentanediol + NADH + H+ low activity Serratia marcescens H30 ?
-
 ?
1.1.1.B20 1,2-propandiol + NADH + H+ low activity Serratia marcescens ?
-
 ?
1.1.1.B20 diacetyl + NADH + H+
-
 Serratia marcescens (3S)-acetoin + NAD+
-
 ?
1.1.1.B20 glycerol + NADH + H+ low activity Serratia marcescens ?
-
 ?
1.1.1.B20 meso-2,3-butanediol + NAD+
-
 Serratia marcescens (3R)-acetoin + NADH + H+
-
 r
1.1.1.B20 additional information (2R,3R)-2,3-butanediol is no substrate for the enzyme Serratia marcescens ?
-
 ?
1.1.1.B20 additional information (2R,3R)-2,3-butanediol is no substrate for the enzyme Serratia marcescens H30 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B20 BDH
-
 Serratia marcescens
1.1.1.B20 NAD(H)-dependent meso-2,3-BDH
-
 Serratia marcescens
1.1.1.B20 NAD(H)-dependent meso-2,3-butanediol dehydrogenase
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B20 40
-
 optimum for oxidation and reduction reactions Serratia marcescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.B20 1.02
-
 (2S,3S)-2,3-butanediol pH 5.0, 40°C Serratia marcescens
1.1.1.B20 6.2
-
 meso-2,3-butanediol pH 5.0, 40°C Serratia marcescens
1.1.1.B20 11.5
-
 diacetyl pH 8.0, 40°C Serratia marcescens
1.1.1.B20 19.7
-
 (3R)-acetoin pH 8.0, 40°C Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B20 5
-
 maximal activity of (3S/3R)-acetoin reduction Serratia marcescens
1.1.1.B20 8
-
 maximal activity of diacetyl reduction and meso-2,3-butanediol oxidation Serratia marcescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B20 NAD+
-
 Serratia marcescens
1.1.1.B20 NADH
-
 Serratia marcescens

General Information

EC Number General Information Comment Organism
1.1.1.B20 evolution the enzyme belongs to the shortchain dehydrogenase/reductase superfamily Serratia marcescens
1.1.1.B20 additional information the enzyme possesses two conserved sequences including the coenzyme binding motif (GxxxGxG) and the active-site motif (YxxxK) Serratia marcescens