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Literature summary extracted from

  • Karsten, W.E.; Reyes, Z.L.; Bobyk, K.D.; Cook, P.F.; Chooback, L.
    Mechanism of the aromatic aminotransferase encoded by the Aro8 gene from Saccharomyces cerevisiae (2011), Arch. Biochem. Biophys., 516, 67-74.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.39 His-tagged protein expressed in Escherichia coli BL21-Codon-Plus(DE3)-RIL Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.39 0.12
-
 2-oxoadipate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
2.6.1.39 0.5
-
 L-tyrosine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
2.6.1.39 1
-
 L-phenylalanine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
2.6.1.39 3.6
-
 2-oxoglutarate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
2.6.1.39 8.8
-
 L-2-aminoadipate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
2.6.1.39 26
-
 L-glutamate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.39 56168
-
 2 * 56168 Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.39 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.39 immobilized metal ion affinity chromatography (Ni2+) Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.39 2-oxoglutarate + L-2-aminoadipate
-
 Saccharomyces cerevisiae 2-oxoadipate + L-glutamate
-
 r
2.6.1.39 2-oxoglutarate + L-tyrosine
-
 Saccharomyces cerevisiae L-glutamate + 4-hydroxyphenylpyruvate
-
 r
2.6.1.39 L-2-aminoadipate + 4-hydroxyphenylpyruvate
-
 Saccharomyces cerevisiae 2-oxoadipate + L-tyrosine
-
 ?
2.6.1.39 L-glutamate + 4-hydroxyphenylpyruvate
-
 Saccharomyces cerevisiae 2-oxoglutarate + L-tyrosine
-
 r
2.6.1.39 L-phenylalanine + 2-oxoglutarate
-
 Saccharomyces cerevisiae phenylpyruvate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.39 homodimer 2 * 56168 Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.6.1.39 alpha-aminoadipate aminotransferase (gene aro8)
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.39 8.3
-
 L-phenylalanine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
2.6.1.39 12.3
-
 L-tyrosine pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
2.6.1.39 12.8
-
 L-glutamate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae
2.6.1.39 18.4
-
 L-2-aminoadipate pH 7.0, temperature not specified in the publication Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.39 6.5 9.6 The V/Et and V/Kalpha-ketoadipateEt pH profiles are pH independent from pH 6.5 to 9.6, while the V/KL-tyrosine pH-rate profile decreases below a single pKa of 7.0 ± 0.1 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.39 pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae