Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Duff, S.M.; Rydel, T.J.; McClerren, A.L.; Zhang, W.; Li, J.Y.; Sturman, E.J.; Halls, C.; Chen, S.; Zeng, J.; Peng, J.; Kretzler, C.N.; Evdokimov, A.
    The enzymology of alanine aminotransferase (AlaAT) isoforms from Hordeum vulgare and other organisms, and the HvAlaAT crystal structure (2012), Arch. Biochem. Biophys., 528, 90-101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.2 expressed in Escherichia coli as a His-tagged fusion protein Saccharomyces cerevisiae
2.6.1.2 expressed in Escherichia coli as a His-tagged fusion protein Zea mays
2.6.1.2 expressed in Escherichia coli as a His-tagged fusion protein Rhodopseudomonas palustris
2.6.1.2 expressed in Escherichia coli as a His-tagged fusion protein Pseudomonas putida
2.6.1.2 expressed in Escherichia coli as a His-tagged fusion protein Oryza sativa
2.6.1.2 expressed in Escherichia coli as a His-tagged fusion protein Halalkalibacterium halodurans
2.6.1.2 expressed in Escherichia coli as a His-tagged fusion protein Hordeum vulgare

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.2 HvAlaAT is crystallized in complex with pyridoxal 5'-phosphate and L-cycloserine. Structure of this complex is solved at 2.7 A resolution Hordeum vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.2 (aminooxy)acetate
-
 Hordeum vulgare
2.6.1.2 (aminooxy)acetate
-
 Saccharomyces cerevisiae
2.6.1.2 cycloserine
-
 Hordeum vulgare
2.6.1.2 cycloserine
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.2 0.05
-
 2-oxoglutarate 25°C, pH 8.0 Pseudomonas putida
2.6.1.2 0.08
-
 pyruvate 25°C, pH 8.0 Rhodopseudomonas palustris
2.6.1.2 0.08
-
 pyruvate 25°C, pH 8.0 Halalkalibacterium halodurans
2.6.1.2 0.11
-
 pyruvate 25°C, pH 8.0 Oryza sativa
2.6.1.2 0.2
-
 pyruvate 25°C, pH 8.0 Pseudomonas putida
2.6.1.2 0.2
-
 pyruvate 25°C, pH 8.0 Hordeum vulgare
2.6.1.2 0.2
-
 2-oxoglutarate 25°C, pH 8.0 Saccharomyces cerevisiae
2.6.1.2 0.2
-
 2-oxoglutarate 25°C, pH 8.0 Zea mays
2.6.1.2 0.2
-
 2-oxoglutarate 25°C, pH 8.0 Rhodopseudomonas palustris
2.6.1.2 0.2
-
 L-glutamate 25°C, pH 8.0 Rhodopseudomonas palustris
2.6.1.2 0.21
-
 L-alanine 25°C, pH 8.0 Pseudomonas putida
2.6.1.2 0.24
-
 L-glutamate 25°C, pH 8.0 Saccharomyces cerevisiae
2.6.1.2 0.25
-
 2-oxoglutarate 25°C, pH 8.0 Oryza sativa
2.6.1.2 0.3
-
 2-oxoglutarate 25°C, pH 8.0 Hordeum vulgare
2.6.1.2 0.32
-
 2-oxoglutarate 25°C, pH 8.0 Halalkalibacterium halodurans
2.6.1.2 0.37
-
 2-oxoglutarate 25°C, pH 8.0 Zea mays
2.6.1.2 0.4
-
 pyruvate 25°C, pH 8.0 Saccharomyces cerevisiae
2.6.1.2 0.4
-
 L-alanine 25°C, pH 8.0 Oryza sativa
2.6.1.2 0.42
-
 pyruvate 25°C, pH 8.0 Zea mays
2.6.1.2 0.44
-
 L-glutamate 25°C, pH 8.0 Halalkalibacterium halodurans
2.6.1.2 0.54
-
 pyruvate 25°C, pH 8.0 Zea mays
2.6.1.2 0.55
-
 L-glutamate 25°C, pH 8.0 Zea mays
2.6.1.2 0.56
-
 L-glutamate 25°C, pH 8.0 Zea mays
2.6.1.2 0.57
-
 L-glutamate 25°C, pH 8.0 Pseudomonas putida
2.6.1.2 0.62
-
 L-alanine 25°C, pH 8.0 Zea mays
2.6.1.2 0.68
-
 L-glutamate 25°C, pH 8.0 Oryza sativa
2.6.1.2 0.8
-
 L-glutamate 25°C, pH 8.0 Hordeum vulgare
2.6.1.2 1.5 2 L-alanine 25°C, pH 8.0 Rhodopseudomonas palustris
2.6.1.2 3.06
-
 L-alanine 25°C, pH 8.0 Zea mays
2.6.1.2 3.6
-
 L-alanine 25°C, pH 8.0 Saccharomyces cerevisiae
2.6.1.2 3.8
-
 L-alanine 25°C, pH 8.0 Hordeum vulgare
2.6.1.2 5.15
-
 L-alanine 25°C, pH 8.0 Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.2 Halalkalibacterium halodurans
-
-
-
2.6.1.2 Hordeum vulgare P52894
-
-
2.6.1.2 Oryza sativa
-
-
-
2.6.1.2 Pseudomonas putida
-
-
-
2.6.1.2 Rhodopseudomonas palustris
-
-
-
2.6.1.2 Saccharomyces cerevisiae
-
-
-
2.6.1.2 Zea mays
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Saccharomyces cerevisiae pyruvate + L-glutamate
-
 r
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Zea mays pyruvate + L-glutamate
-
 r
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Rhodopseudomonas palustris pyruvate + L-glutamate
-
 r
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Pseudomonas putida pyruvate + L-glutamate
-
 r
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Oryza sativa pyruvate + L-glutamate
-
 r
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Halalkalibacterium halodurans pyruvate + L-glutamate
-
 r
2.6.1.2 L-alanine + 2-oxoglutarate
-
 Hordeum vulgare pyruvate + L-glutamate
-
 r
2.6.1.2 additional information enzyme is also able to utilize aspartate and oxaloacetate with 10% efficiency as compared to the native substrates Hordeum vulgare ?
-
 ?
2.6.1.2 pyruvate + L-glutamate
-
 Hordeum vulgare L-alanine + 2-oxoglutarate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.2 Bh D-AlaAT
-
 Halalkalibacterium halodurans
2.6.1.2 HvAlaAT
-
 Hordeum vulgare
2.6.1.2 OsAlaAT
-
 Oryza sativa
2.6.1.2 Pp beta-AlaAT
-
 Pseudomonas putida
2.6.1.2 Rp D-AlaAT
-
 Rhodopseudomonas palustris
2.6.1.2 ScAlaAT
-
 Saccharomyces cerevisiae
2.6.1.2 ZmAlaAT1
-
 Zea mays
2.6.1.2 ZmAlaAT2
-
 Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.2 0.08
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Pseudomonas putida
2.6.1.2 0.08
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Pseudomonas putida
2.6.1.2 0.17
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Pseudomonas putida
2.6.1.2 0.17
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Pseudomonas putida
2.6.1.2 0.33
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Zea mays
2.6.1.2 0.33
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Zea mays
2.6.1.2 0.38
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Rhodopseudomonas palustris
2.6.1.2 0.38
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Rhodopseudomonas palustris
2.6.1.2 0.67
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Zea mays
2.6.1.2 0.67
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Zea mays
2.6.1.2 1.25
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Halalkalibacterium halodurans
2.6.1.2 1.25
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Halalkalibacterium halodurans
2.6.1.2 2.8
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Halalkalibacterium halodurans
2.6.1.2 2.8
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Halalkalibacterium halodurans
2.6.1.2 3.06
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Rhodopseudomonas palustris
2.6.1.2 3.06
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Rhodopseudomonas palustris
2.6.1.2 4.98
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Oryza sativa
2.6.1.2 4.98
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Oryza sativa
2.6.1.2 5.49
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Zea mays
2.6.1.2 5.49
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Zea mays
2.6.1.2 6.09
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Saccharomyces cerevisiae
2.6.1.2 6.09
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Saccharomyces cerevisiae
2.6.1.2 6.56
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Zea mays
2.6.1.2 6.56
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Zea mays
2.6.1.2 6.86
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Oryza sativa
2.6.1.2 6.86
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Oryza sativa
2.6.1.2 8.64
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Saccharomyces cerevisiae
2.6.1.2 8.64
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Saccharomyces cerevisiae
2.6.1.2 12.11
-
 L-alanine cofactor: 2-oxoglutarate, 25°C, pH 8.0 Hordeum vulgare
2.6.1.2 12.11
-
 2-oxoglutarate cofactor: L-alanine, 25°C, pH 8.0 Hordeum vulgare
2.6.1.2 25.62
-
 pyruvate cofactor: L-glutamate, 25°C, pH 8.0 Hordeum vulgare
2.6.1.2 25.62
-
 L-glutamate cofactor: pyruvate, 25°C, pH 8.0 Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.2 pyridoxal 5'-phosphate
-
 Saccharomyces cerevisiae
2.6.1.2 pyridoxal 5'-phosphate
-
 Zea mays
2.6.1.2 pyridoxal 5'-phosphate
-
 Rhodopseudomonas palustris
2.6.1.2 pyridoxal 5'-phosphate
-
 Pseudomonas putida
2.6.1.2 pyridoxal 5'-phosphate
-
 Oryza sativa
2.6.1.2 pyridoxal 5'-phosphate
-
 Halalkalibacterium halodurans
2.6.1.2 pyridoxal 5'-phosphate
-
 Hordeum vulgare

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.6.1.2 0.0069
-
 25°C, pH 8.0 Hordeum vulgare (aminooxy)acetate
2.6.1.2 0.119
-
 25°C, pH 8.0 Hordeum vulgare cycloserine