Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Steen, I.H.; Lien, T.; Madsen, M.S.; Birkeland, N.K.
    Identification of cofactor discrimination sites in NAD-isocitrate dehydrogenase from Pyrococcus furiosus (2002), Arch. Microbiol., 178, 297-300.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.41 D328K the mutant shows dual coenzyme specificity Pyrococcus furiosus
1.1.1.41 D328K/I329Y introduction of the double mutation shifts the cofactor preference from NAD+ to NADP+ Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 0.066
-
 NAD+ pH 8.0, 70°C, mutant enzyme D328K Pyrococcus furiosus
1.1.1.41 0.068
-
 NAD+ pH 8.0, 70°C, wild-type enzyme Pyrococcus furiosus
1.1.1.41 0.162
-
 NADP+ pH 8.0, 70°C, mutant enzyme D328K Pyrococcus furiosus
1.1.1.41 0.211
-
 NADP+ pH 8.0, 70°C, mutant enzyme D328K/I329Y Pyrococcus furiosus
1.1.1.41 0.336
-
 NAD+ pH 8.0, 70°C, mutant enzyme D328K/I329Y Pyrococcus furiosus
1.1.1.41 4.44
-
 NADP+ pH 8.0, 70°C, wild-type enzyme Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Pyrococcus furiosus Q8U488
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+ wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ Pyrococcus furiosus 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NADP+ wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ Pyrococcus furiosus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 NAD-IDH
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.41 70
-
 assay at Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.41 80 95 activity at 80°C is about 50% compared to the activity at 90°C Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.41 102
-
 the apparent melting temperature is 102.3°C, mutant enzyme D328K Pyrococcus furiosus
1.1.1.41 103
-
 the apparent melting temperature is 102.9°C, mutant enzyme D328K/I329Y Pyrococcus furiosus
1.1.1.41 104
-
 the apparent melting temperature is 103.7°C, wild-type enzyme Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.41 60.4
-
 NAD+ pH 8.0, 70°C, wild-type enzyme Pyrococcus furiosus
1.1.1.41 93
-
 NADP+ pH 8.0, 70°C, wild-type enzyme Pyrococcus furiosus
1.1.1.41 103.4
-
 NAD+ pH 8.0, 70°C, mutant enzyme D328K Pyrococcus furiosus
1.1.1.41 105.6
-
 NAD+ pH 8.0, 70°C, mutant enzyme D328K/I329Y Pyrococcus furiosus
1.1.1.41 133
-
 NADP+ pH 8.0, 70°C, mutant enzyme D328K Pyrococcus furiosus
1.1.1.41 168
-
 NADP+ pH 8.0, 70°C, mutant enzyme D328K/I329Y Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.41 8
-
 assay at Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+ wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ Pyrococcus furiosus
1.1.1.41 NADP+ wild-type enzyme is specific for NAD+, low activity with NADP+ as cofactor, mutant D328K shows dual coenzyme specificity, cofactor preference of mutant D328K/I329Y is shifted from NAD+ to NADP+ Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.41 20.9
-
 NADP+ pH 8.0, 70°C, wild-type enzyme Pyrococcus furiosus
1.1.1.41 314
-
 NAD+ pH 8.0, 70°C, mutant enzyme D328K/I329Y Pyrococcus furiosus
1.1.1.41 796
-
 NADP+ pH 8.0, 70°C, mutant enzyme D328K/I329Y Pyrococcus furiosus
1.1.1.41 821
-
 NADP+ pH 8.0, 70°C, mutant enzyme D328K Pyrococcus furiosus
1.1.1.41 888
-
 NAD+ pH 8.0, 70°C, wild-type enzyme Pyrococcus furiosus
1.1.1.41 1567
-
 NAD+ pH 8.0, 70°C, mutant enzyme D328K Pyrococcus furiosus