Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Petreikov, M.; Eisenstein, M.; Yeselson, Y.; Preiss, J.; Schaffer, A.A.
    Characterization of the AGPase large subunit isoforms from tomato indicates that the recombinant L3 subunit is active as a monomer (2010), Biochem. J., 428, 201-212.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 3-phosphoglycerate activation of the heterotetramer comprised of large subunit isoform L1 and small subunit S is almost completely dependent on 3-phosphoglycerate. The heterotetramer comprised of large subunit isoform L3 and small subunit S shows approx. 20% of its maximal activity even in the absence of 3-phosphoglycerate Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.27 expression in Escherichia coli Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.27 additional information expression of the large subunit isoforms L1 to L3 in Escherichia coli, in conjunction with small subunit S and individually and kinetic characterisation of the L1/S and L3/S heterotetramers. The recombinant L3 subunit is also active when expressed alone Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.08
-
 diphosphate pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S Solanum lycopersicum
2.7.7.27 0.12
-
 diphosphate pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S Solanum lycopersicum
2.7.7.27 0.29
-
 alpha-D-glucose 1-phosphate pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S Solanum lycopersicum
2.7.7.27 0.34
-
 ATP pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S Solanum lycopersicum
2.7.7.27 0.41
-
 diphosphate pH 7.8, 37°C, large subunit isoform L3 Solanum lycopersicum
2.7.7.27 0.45
-
 ADP-glucose pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L1 and small subunit S Solanum lycopersicum
2.7.7.27 0.46
-
 alpha-D-glucose 1-phosphate pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S Solanum lycopersicum
2.7.7.27 0.5
-
 ATP pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S Solanum lycopersicum
2.7.7.27 0.72
-
 ADP-glucose pH 7.8, 37°C, heterotetramer comprised of large subunit isoform L3 and small subunit S Solanum lycopersicum
2.7.7.27 1.57
-
 ADP-glucose pH 7.8, 37°C, large subunit isoform L3 Solanum lycopersicum
2.7.7.27 2.1
-
 alpha-D-glucose 1-phosphate pH 7.8, 37°C, large subunit isoform L3 Solanum lycopersicum
2.7.7.27 3.6
-
 ATP pH 7.8, 37°C, large subunit isoform L3 Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Solanum lycopersicum Q15I66 large subunit isoform L1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.27 partial Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.27 fruit
-
 Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Solanum lycopersicum diphosphate + ADP-glucose
-
 r
2.7.7.27 diphosphate + ADP-glucose
-
 Solanum lycopersicum ATP + alpha-D-glucose 1-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.27 AGPase
-
 Solanum lycopersicum