Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Owen, J.G.; Copp, J.N.; Ackerley, D.F.
    Rapid and flexible biochemical assays for evaluating 4-phosphopantetheinyl transferase activity (2011), Biochem. J., 436, 709-717.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis
2.7.8.7 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa
2.7.8.7 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.7 6-nitroso-1,2-benzopyrone
-
Bacillus subtilis
2.7.8.7 6-nitroso-1,2-benzopyrone
-
Pseudomonas aeruginosa
2.7.8.7 6-nitroso-1,2-benzopyrone
-
Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.00062
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
2.7.8.7 0.0033
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
2.7.8.7 0.0038
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
2.7.8.7 0.0065
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
2.7.8.7 0.0086
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
2.7.8.7 0.011
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+ required for activity Bacillus subtilis
2.7.8.7 Mg2+ required for activity Pseudomonas aeruginosa
2.7.8.7 Mg2+ required for activity Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Bacillus subtilis
-
-
-
2.7.8.7 Bacillus subtilis ATCC 6633
-
-
-
2.7.8.7 Pseudomonas aeruginosa
-
-
-
2.7.8.7 Pseudomonas putida
-
-
-
2.7.8.7 Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 Ni-NTA column chromatography Bacillus subtilis
2.7.8.7 Ni-NTA column chromatography Pseudomonas aeruginosa
2.7.8.7 Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
Pseudomonas aeruginosa adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
Pseudomonas putida adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
Pseudomonas putida KT 2240 adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[BpsA protein]
-
Bacillus subtilis ATCC 6633 adenosine 3',5'-bisphosphate + holo-[BpsA protein]
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 4-phosphopantetheinyl transferase
-
Bacillus subtilis
2.7.8.7 4-phosphopantetheinyl transferase
-
Pseudomonas aeruginosa
2.7.8.7 4-phosphopantetheinyl transferase
-
Pseudomonas putida
2.7.8.7 PcpS
-
Pseudomonas aeruginosa
2.7.8.7 PP1183
-
Pseudomonas putida
2.7.8.7 PPTase
-
Bacillus subtilis
2.7.8.7 PPTase
-
Pseudomonas aeruginosa
2.7.8.7 PPTase
-
Pseudomonas putida
2.7.8.7 Sfp
-
Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.7 0.0027
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
2.7.8.7 0.035
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
2.7.8.7 0.037
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
2.7.8.7 0.075
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
2.7.8.7 0.183
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
2.7.8.7 0.683
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.8.7 4'-phosphopantetheine
-
Bacillus subtilis
2.7.8.7 4'-phosphopantetheine
-
Pseudomonas aeruginosa
2.7.8.7 4'-phosphopantetheine
-
Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.8.7 0.00042
-
6-nitroso-1,2-benzopyrone in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
2.7.8.7 0.00053
-
6-nitroso-1,2-benzopyrone in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
2.7.8.7 0.0024
-
6-nitroso-1,2-benzopyrone in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
2.7.8.7 0.003
-
6-nitroso-1,2-benzopyrone in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
2.7.8.7 0.003
-
6-nitroso-1,2-benzopyrone in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
2.7.8.7 0.0052
-
6-nitroso-1,2-benzopyrone in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.8.7 0.0021
-
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis 6-nitroso-1,2-benzopyrone
2.7.8.7 0.0037
-
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida 6-nitroso-1,2-benzopyrone
2.7.8.7 0.004
-
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa 6-nitroso-1,2-benzopyrone
2.7.8.7 0.0091
-
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis 6-nitroso-1,2-benzopyrone
2.7.8.7 0.0098
-
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida 6-nitroso-1,2-benzopyrone
2.7.8.7 0.0108
-
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa 6-nitroso-1,2-benzopyrone

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.8.7 3.3
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
2.7.8.7 3.5
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis
2.7.8.7 20
-
CoA-[4'-phosphopantetheine] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
2.7.8.7 21.7
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas putida
2.7.8.7 107
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Pseudomonas aeruginosa
2.7.8.7 108
-
apo-[BpsA protein] in 100 mM Tris-HCl, pH 7.8, at 30°C Bacillus subtilis