EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.7 | expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
2.7.8.7 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
2.7.8.7 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.7 | 6-nitroso-1,2-benzopyrone | - |
Bacillus subtilis | |
2.7.8.7 | 6-nitroso-1,2-benzopyrone | - |
Pseudomonas aeruginosa | |
2.7.8.7 | 6-nitroso-1,2-benzopyrone | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.8.7 | 0.00062 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
2.7.8.7 | 0.0033 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
2.7.8.7 | 0.0038 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
2.7.8.7 | 0.0065 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
2.7.8.7 | 0.0086 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
2.7.8.7 | 0.011 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.7 | Mg2+ | required for activity | Bacillus subtilis | |
2.7.8.7 | Mg2+ | required for activity | Pseudomonas aeruginosa | |
2.7.8.7 | Mg2+ | required for activity | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.7 | Bacillus subtilis | - |
- |
- |
2.7.8.7 | Bacillus subtilis ATCC 6633 | - |
- |
- |
2.7.8.7 | Pseudomonas aeruginosa | - |
- |
- |
2.7.8.7 | Pseudomonas putida | - |
- |
- |
2.7.8.7 | Pseudomonas putida KT 2240 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.7 | Ni-NTA column chromatography | Bacillus subtilis |
2.7.8.7 | Ni-NTA column chromatography | Pseudomonas aeruginosa |
2.7.8.7 | Ni-NTA column chromatography | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.7 | CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? | |
2.7.8.7 | CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Pseudomonas aeruginosa | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? | |
2.7.8.7 | CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Pseudomonas putida | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? | |
2.7.8.7 | CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Pseudomonas putida KT 2240 | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? | |
2.7.8.7 | CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Bacillus subtilis ATCC 6633 | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.7 | 4-phosphopantetheinyl transferase | - |
Bacillus subtilis |
2.7.8.7 | 4-phosphopantetheinyl transferase | - |
Pseudomonas aeruginosa |
2.7.8.7 | 4-phosphopantetheinyl transferase | - |
Pseudomonas putida |
2.7.8.7 | PcpS | - |
Pseudomonas aeruginosa |
2.7.8.7 | PP1183 | - |
Pseudomonas putida |
2.7.8.7 | PPTase | - |
Bacillus subtilis |
2.7.8.7 | PPTase | - |
Pseudomonas aeruginosa |
2.7.8.7 | PPTase | - |
Pseudomonas putida |
2.7.8.7 | Sfp | - |
Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.8.7 | 0.0027 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
2.7.8.7 | 0.035 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
2.7.8.7 | 0.037 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
2.7.8.7 | 0.075 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
2.7.8.7 | 0.183 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
2.7.8.7 | 0.683 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.7 | 4'-phosphopantetheine | - |
Bacillus subtilis | |
2.7.8.7 | 4'-phosphopantetheine | - |
Pseudomonas aeruginosa | |
2.7.8.7 | 4'-phosphopantetheine | - |
Pseudomonas putida |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.8.7 | 0.00042 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
2.7.8.7 | 0.00053 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
2.7.8.7 | 0.0024 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
2.7.8.7 | 0.003 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
2.7.8.7 | 0.003 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
2.7.8.7 | 0.0052 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.8.7 | 0.0021 | - |
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | 6-nitroso-1,2-benzopyrone | |
2.7.8.7 | 0.0037 | - |
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | 6-nitroso-1,2-benzopyrone | |
2.7.8.7 | 0.004 | - |
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | 6-nitroso-1,2-benzopyrone | |
2.7.8.7 | 0.0091 | - |
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | 6-nitroso-1,2-benzopyrone | |
2.7.8.7 | 0.0098 | - |
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | 6-nitroso-1,2-benzopyrone | |
2.7.8.7 | 0.0108 | - |
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | 6-nitroso-1,2-benzopyrone |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.8.7 | 3.3 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
2.7.8.7 | 3.5 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
2.7.8.7 | 20 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
2.7.8.7 | 21.7 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
2.7.8.7 | 107 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
2.7.8.7 | 108 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis |