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Literature summary extracted from

  • Shen, B.; Hutchinson, C.R.
    Tetracenomycin F1 monooxygenase: oxidation of a naphthacenone to a naphthacenequinone in the biosynthesis of tetracenomycin C in Streptomyces glaucescens (1993), Biochemistry, 32, 6656-6663.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.21 CuCl2 31% loss of activity Streptomyces glaucescens
1.13.12.21 diethyl dicarbonate
-
 Streptomyces glaucescens
1.13.12.21 N-ethylmaleimide 0.025 mM, complete inhibition Streptomyces glaucescens
1.13.12.21 p-chloromercuribenzoate 0.025 mM, complete inhibition Streptomyces glaucescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.21 0.00747
-
 tetracenomycin F1 pH 7.5, 30°C Streptomyces glaucescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.21 additional information does not use a metal ion Streptomyces glaucescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.21 12500
-
 3 * 12500, SDS-PAGE Streptomyces glaucescens
1.13.12.21 37500
-
 gel filtration Streptomyces glaucescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.21 tetracenomycin F1 + O2 Streptomyces glaucescens the enzyme is involved in the biosynthesis of tetracenomycin C tetracenomycin D3 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.21 Streptomyces glaucescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.21
-
 Streptomyces glaucescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.21 0.599
-
 pH 7.5, 30°C Streptomyces glaucescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.21 tetracenomycin F1 + O2 the enzyme is involved in the biosynthesis of tetracenomycin C Streptomyces glaucescens tetracenomycin D3 + H2O
-
 ?
1.13.12.21 tetracenomycin F1 + O2 i.e. 3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-6,11-dihydrotetracene-2-carboxylate = 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-6,11-dioxo-2-naphthacenecarboxylic acid Streptomyces glaucescens tetracenomycin D3 + H2O i.e. 3,8,10,12-tetrahydroxy-1-methyl-11-oxo-6,11-dihydro-2-tetracenecarboxylate, i.e. 6,11-dihydro-3,8,10,12-tetrahydroxy-1-methyl-11-oxo-2-naphthacenecarboxylic acid ?

Subunits

EC Number Subunits Comment Organism
1.13.12.21 trimer 3 * 12500, SDS-PAGE Streptomyces glaucescens

Synonyms

EC Number Synonyms Comment Organism
1.13.12.21 TcmF1 monooxygenase
-
 Streptomyces glaucescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.21 7.5
-
-
 Streptomyces glaucescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.21 7 8 pH 7.0: about 40% of maximal activity, pH 8.0: about 85% of maximal activity Streptomyces glaucescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.21 additional information does not possess a prosthetic group such as flavin or heme Streptomyces glaucescens

General Information

EC Number General Information Comment Organism
1.13.12.21 physiological function the enzyme is involved in the biosynthesis of tetracenomycin C Streptomyces glaucescens