EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.48 | expression in Escherichia coli | Saccharolobus solfataricus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.1.48 | mutagenesis data and crystal structure analysis of IGPS from Sulfolobus solfataricus allows for the formulation of a plausible chemical mechanism of the reaction | Saccharolobus solfataricus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.48 | D61C | kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is about 1.5fold lower than the wild-type value | Saccharolobus solfataricus |
4.1.1.48 | R18C | kcat/Km for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate is about 1.5fold lower than the wild-type value | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.48 | 0.000045 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye Alexa Fluor 555 | Saccharolobus solfataricus | |
4.1.1.48 | 0.000046 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye PyMPO | Saccharolobus solfataricus | |
4.1.1.48 | 0.00005 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye PyMPO | Saccharolobus solfataricus | |
4.1.1.48 | 0.000051 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, wild-type enzyme | Saccharolobus solfataricus | |
4.1.1.48 | 0.000109 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye Alexa Fluor 555 | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | Saccharolobus solfataricus | the enzyme is involved in tryptophan biosynthesis | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | Saccharolobus solfataricus P2 | the enzyme is involved in tryptophan biosynthesis | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.48 | Saccharolobus solfataricus | Q06121 | - |
- |
4.1.1.48 | Saccharolobus solfataricus P2 | Q06121 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.48 | - |
Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | the enzyme is involved in tryptophan biosynthesis | Saccharolobus solfataricus | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | elucidation of the kinetic reaction mechanism, importance of flexible active loops for substrate binding and catalysis by the enzyme, product release is the rate-limiting step of the overall reaction | Saccharolobus solfataricus | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | the enzyme is involved in tryptophan biosynthesis | Saccharolobus solfataricus P2 | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | elucidation of the kinetic reaction mechanism, importance of flexible active loops for substrate binding and catalysis by the enzyme, product release is the rate-limiting step of the overall reaction | Saccharolobus solfataricus P2 | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.48 | sIGPS | - |
Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.48 | 25 | - |
assay at | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.48 | 0.038 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye PyMPO | Saccharolobus solfataricus | |
4.1.1.48 | 0.04 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye Alexa Fluor 555 | Saccharolobus solfataricus | |
4.1.1.48 | 0.042 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye PyMPO | Saccharolobus solfataricus | |
4.1.1.48 | 0.062 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, wild-type enzyme | Saccharolobus solfataricus | |
4.1.1.48 | 0.065 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye Alexa Fluor 555 | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.48 | 7.5 | - |
assay | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.48 | 596 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye Alexa Fluor 555 | Saccharolobus solfataricus | |
4.1.1.48 | 826 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme D61C labeled with the fluorescent dye PyMPO | Saccharolobus solfataricus | |
4.1.1.48 | 840 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye PyMPO | Saccharolobus solfataricus | |
4.1.1.48 | 888 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, mutant enzyme R18C labeled with the fluorescent dye Alexa Fluor 555 | Saccharolobus solfataricus | |
4.1.1.48 | 1215 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | pH 7.5, 25°C, wild-type enzyme | Saccharolobus solfataricus |