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Literature summary extracted from

  • Jeon, W.Y.; Yoon, B.H.; Ko, B.S.; Shim, W.Y.; Kim, J.H.
    Xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene in Candida tropicalis (2012), Bioprocess Biosyst. Eng., 35, 191-198.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.307 additional information xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene under control of a constitutive glyceraldehyde-3-phosphate dehydrogenase promoter in a Candida tropicalis xylitol dehydrogenase gene (XYL2)-disrupted strain resulting in recombinant strain LNG2, overview Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.307 xylitol + NAD+ Candida tropicalis
-
 D-xylose + NADH + H+
-
 r
1.1.1.307 xylitol + NAD+ Candida tropicalis ATCC 20336
-
 D-xylose + NADH + H+
-
 r
1.1.1.307 xylitol + NADP+ Candida tropicalis
-
 D-xylose + NADPH + H+
-
 r
1.1.1.307 xylitol + NADP+ Candida tropicalis ATCC 20336
-
 D-xylose + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.307 Candida tropicalis
-
-
-
1.1.1.307 Candida tropicalis ATCC 20336
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.307 2.86
-
 recombinant strain LNG2, pH 7.0, 25°C, xylose reduction with NADPH Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.307 xylitol + NAD+
-
 Candida tropicalis D-xylose + NADH + H+
-
 r
1.1.1.307 xylitol + NAD+
-
 Candida tropicalis ATCC 20336 D-xylose + NADH + H+
-
 r
1.1.1.307 xylitol + NADP+
-
 Candida tropicalis D-xylose + NADPH + H+
-
 r
1.1.1.307 xylitol + NADP+
-
 Candida tropicalis ATCC 20336 D-xylose + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.307 xylose reductase
-
 Candida tropicalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.307 25
-
 assay at Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.307 7
-
 assay at Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.307 NAD+
-
 Candida tropicalis
1.1.1.307 NADH
-
 Candida tropicalis
1.1.1.307 NADP+
-
 Candida tropicalis
1.1.1.307 NADPH
-
 Candida tropicalis

Expression

EC Number Organism Comment Expression
1.1.1.307 Candida tropicalis formation of xylose reductase in the yeast Candida tropicalis is significantly repressed in cells grown on medium that contains glucose as carbon and energy source, because of the repressive effect of glucose down

General Information

EC Number General Information Comment Organism
1.1.1.307 metabolism xylose reductase is the first enzyme in D-xylose metabolism, catalyzing the reduction of D-xylose to xylitol Candida tropicalis